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determination of antimicrobial activity of sorrel (hibiscus sabdariffa) on esherichia coli o157:h7 isolated from food, veterinary, and clinical samples.abstract the use of medicinal plants as natural antimicrobial agents is gaining popularity. sorrel (hibiscus sabdariffa) is widely used for the treatment of diseases. the objective of this study was to investigate the antimicrobial activity of sorrel on escherichia coli o157:h7 isolates from food, veterinary, and clinical samples. phenolics of the calyces were extracted from 10 g of ground, freeze-dried samples using 100 ml of 80% aqueous methanol. concentrations of 10%, 5%, and 2.5% methanol ex ...201121548802
characterization and survival of environmental escherichia coli o26 isolates in ground beef and environmental samples.in addition to escherichia coli o157:h7, shiga toxin-producing e. coli (stec) o26 was added to the zero-tolerance adulterant list together with other 5 non-o157 stec serogroups in 2012. four farm o26 isolates were used in this study; they were obtained from a on-farm survey study conducted in alabama. the presence of 3 major pathogenic genes (stx1, stx2, and eaea) was determined through multiplex polymerase chain reaction (pcr). two major pathogenic gene profiles were observed: 3 of the farm iso ...201525765176
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