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first case of granulomatous amebic encephalitis caused by acanthamoeba castellanii in taiwan.we report the first case of granulomatous amebic encephalitis caused by acanthamoeba in a previously healthy farmer in taiwan who fell into a ditch. the dna amplicons of amebas identified by polymerase chain reaction in the cerebrospinal fluid specimen and the ditch water specimens were identical and shared strong homology with a. castellanii . he survived after treatment with amphotericin b, rifampin, and corticosteroids.200919635883
characterizing clinical isolates of acanthamoeba castellanii with high resistance to polyhexamethylene biguanide in taiwan.acanthamoeba keratitis (ak), a painful infectious corneal disease, is caused by the free-living pathogenic species acanthamoeba. the symptoms include corneal infiltrate, epithelial, and stromal destruction, and loss of vision. current treatment generally involves an hourly application of polyhexamethylene biguanide (phmb) over a period of several days; however, even this is not entirely effective against all strains/isolates. the aims of this study were to confirm the existence of pathogenic str ...201526698685
identification and quantification of the acanthamoeba species and genotypes from reservoirs in taiwan by molecular techniques.the occurrence of acanthamoeba was investigated from 21 main reservoirs of taiwan with 12 (57.1%) testing positive. analysis of the 18s rrna gene pcr product was performed in order to identify the acanthamoeba isolates. acanthamoeba spp. concentrations were determined according to taqman real-time qpcr. acanthamoeba genotypes of all isolates were identified t4. the species were categorized to acanthamoeba culbertsoni, acanthamoeba polyphaga, acanthamoeba castellanii and acanthamoeba hatchetti. t ...201424388954
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