| reproducibility and quantitation of amplicon sequencing-based detection. | to determine the reproducibility and quantitation of the amplicon sequencing-based detection approach for analyzing microbial community structure, a total of 24 microbial communities from a long-term global change experimental site were examined. genomic dna obtained from each community was used to amplify 16s rrna genes with two or three barcode tags as technical replicates in the presence of a small quantity (0.1% wt/wt) of genomic dna from shewanella oneidensis mr-1 as the control. the techni ... | 2011 | 21346791 |