| the correct sequence of the porcine group c/cowden rotavirus major inner capsid protein shows close homology with human isolates from brazil and the u.k. | amino acid sequence alignments between the human group c/bristol and the published porcine group c/cowden vp6 proteins have revealed a region of extreme sequence divergence. we have been unable to confirm the nucleotide sequence of the cowden vp6 gene corresponding to this region of divergence. direct sequencing of a pcr-amplified cdna pool has revealed a frame shift, and three nucleotide changes, within the published sequence of the porcine (cowden) vp6 gene. the corrected sequence of the porci ... | 1992 | 1326817 |
| atypical rotavirus among diarrhoeic children living in belém, brazil. | atypical rotaviruses were detected in faeces from two diarrhoeic children living in belém, pará, brazil. rotavirus particles were detected by electron microscopy and the rna electrophoresis showed patterns which were compatible with group c rotaviruses. tests for the presence of group a antigen by enzyme-linked-immunosorbent assay (elisa) were negative. the two children had three successive rotavirus infections and in both cases the atypical strains were excreted at the time of the third infecti ... | 1989 | 2157132 |
| molecular characterization of the outer capsid spike protein (vp4) gene from human group c rotavirus. | a cdna copy of the third genomic rna segment of a noncultivatable human group c rotavirus (bristol) was generated by single primer amplification. human group c rotavirus genome segment 3 contains 2283 bp and encodes the vp4 gene with an open reading frame of 2232 nucleotides (744 amino acids) starting at nucleotide 21 and terminating at nucleotide 2251. pcr primers designed from the 5' and 3' terminal sequences of the c/bristol vp4 gene were used to amplify the corresponding vp4 gene of a human ... | 1994 | 8091676 |
| national laboratory surveillance of viral agents of gastroenteritis in brazil. | in 1984 an enzyme immunoassay for rotavirus and adenovirus developed in the virology department of the oswaldo cruz foundation was distributed to laboratories in 14 brazilian states as part of a project to survey viral agents in fecal specimens from children with diarrhea. the ensuing surveillance continued for 3 to 5 years, and in 1991 the results obtained by all laboratories that tested 300 or more fecal specimens were reviewed at a workshop held in belém. these results showed that rotavirus h ... | 1993 | 8220517 |
| an outbreak of group c rotavirus gastroenteritis among children attending a day-care centre in belém, brazil. | in august 1993, an outbreak of group c rotavirus-associated gastroenteritis occurred among children attending a day-care centre in belém, brazil. of the 64 children, 21 (33%) became ill. group c rotavirus was identified in faecal specimens from 8 (38%) children with diarrhoea by electron microscopy (em) and an enzyme immunoassay (eia), using antibodies specific to the cowden strain of porcine group c rotavirus. by polyacrylamide gel electrophoresis (page), a pattern similar to that of group c ro ... | 1999 | 10897889 |
| evidence for zoonotic transmission of group c rotaviruses among children in belém, brazil. | the prevalence and potential zoonotic transmission of group c rotavirus (rvc) were examined by testing fecal samples collected from children during a longitudinal study that was carried out in the outskirts of belém, brazil, from december 1982 to march 1986. the study involved a group of 30 children who were followed from birth to 3 years. of the 77 samples tested from 29 children, 5 (6.5%) were positive for human and 3 (4%) for porcine rvc by using nested pcr assay with primers specific for vp6 ... | 2008 | 18649333 |