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molecular analysis of outer capsid glycoprotein (vp7) genes from two isolates of human group c rotavirus with different genome electropherotypes.nucleotide sequences for the vp7 gene of human group c rotavirus were determined for two strains isolated in okayama, japan, during a 1988-1990 epidemic. these isolates, ok118 and ok450, were selected as prototypes of two different electropherotypes, patterns i and ii, respectively. the genes were identical in size (1,063 bp), and both contained singled open reading frames encoding 332 amino acids. the alignment of two sequences revealed 46 nucleotide substitutions, 11 of which were predicted to ...19968940469
seroepidemiology of human group c rotavirus in japan based on a blocking enzyme-linked immunosorbent assay.a novel blocking enzyme-linked immunosorbent assay (bl-elisa) was developed for detection of antibodies to human group c rotavirus (chrv). the specificity of the bl-elisa was confirmed by using animal sera hyperimmunized to group a and group c rotaviruses and paired sera from five patients with acute chrv gastroenteritis. furthermore, there was concordance between the bl-elisa and a neutralization assay for chrv in 226 (95%) of 238 samples. by using the bl-elisa, we determined the seroprevalence ...200111139211
an outbreak of acute gastroenteritis caused by human group c rotavirus in a welfare institution in okayama prefecture. 200516116266
detection and molecular characterization of human group c rotaviruses in okayama prefecture, japan, between 1986 and 2005.a survey of human group c rotaviruses (chrvs) was conducted in okayama prefecture, japan, over a period of 19 years between 1986 and 2005. the presence of chrvs was screened by enzyme-linked immunosorbent assay using chrv-specific monoclonal antibodies and confirmed by reverse transcription-pcr. of the 3,722 fecal specimens from sporadic cases of gastroenteritis, 44 specimens (1.2%) were positive for chrv. the chrv isolates were detected periodically but continuously, and the rates of positivity ...200717596825
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