| prokaryotic expression and identification of 3-1e gene of merozoite surface antigen of eimeria acervulina. | eimeria acervulina was isolated from chicken at hebei province, china. the gene of merozoite surface antigen 3-1e was amplified and cloned into pet28a(+) vector and then transformed into escherichia coli bl21 strain. results showed that 3-1e fusion protein band of about 22 kda was identified by sds-page. western blot analysis indicated that the recombinant protein specifically reacted with e. acervulina polyclonal antibody. | 2011 | 21499749 |