| a combined microscopic-molecular method for the diagnosis of strongylid infections in sheep. | we evaluated a combined microscopic-molecular approach for the diagnosis of key strongylid infections in sheep using panels of well-defined control and test samples. the method established is based on the separation of nematode eggs from faecal samples using a salt flotation procedure, the extraction and column-purification of genomic dna, followed by real-time pcr and melting-curve analysis. specific and semi-quantitative amplification from (a minimum of 0.1-2.0pg) genomic dna of haemonchus con ... | 2009 | 19328802 |