| a highly sensitive and specific real-time pcr assay for the detection of spotted fever and typhus group rickettsiae. | a highly specific real-time polymerase chain reaction (pcr) assay was developed to detect spotted fever and typhus group rickettsiae using the citrate synthase gene as the target. the assay amplified rickettsial members of the spotted fever and typhus group including rickettsia akari, r. australis, r. conorii, r. honei, "r. marmionii," r. sibirica, r. rickettsii, r. typhi, and r. prowazekii. the ancestral group rickettsia, r. bellii, did not produce a positive reaction, nor did other members of ... | 2005 | 16354816 |