| detection of rna from a novel west nile-like virus and high prevalence of an insect-specific flavivirus in mosquitoes in the yucatan peninsula of mexico. | as part of our ongoing surveillance efforts for west nile virus (wnv) in the yucatan peninsula of mexico, 96,687 mosquitoes collected from january through december 2007 were assayed by virus isolation in mammalian cells. three mosquito pools caused cytopathic effect. two isolates were orthobunyaviruses (cache valley virus and kairi virus) and the identity of the third infectious agent was not determined. a subset of mosquitoes was also tested by reverse transcription-polymerase chain reaction (r ... | 2009 | 19141845 |
| detection of flaviviruses and orthobunyaviruses in mosquitoes in the yucatan peninsula of mexico in 2008. | a total of 191,244 mosquitoes from 24 species were collected in the yucatan peninsula of mexico from january to december 2008, and tested for the presence of cytopathic virus by virus isolation in vero cells. eighteen virus isolates were obtained, all of which were orthobunyaviruses. these were identified by reverse transcription-polymerase chain reaction (rt-pcr) and nucleotide sequencing as cache valley virus (n=17) and south river virus (n=1). a subset (n=20,124) of culex quinquefasciatus col ... | 2010 | 20370430 |
| complete nucleotide sequences of the small and medium rna genome segments of kairi virus (family bunyaviridae). | we determined the complete nucleotide sequences of the small (s) and medium (m) rna genome segments of a kairi virus (kriv) isolate from the yucatan peninsula of mexico. the s segment consists of 992 nucleotides, and the m segment consists of 4,619 nucleotides. phylogenetic analyses were conducted on each genomic segment, and these data are discussed. a 526 nucleotide region of the large (l) segment was also sequenced. this is the first study to present sequence and phylogenetic data for a kriv ... | 2009 | 19649762 |