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a comparison of serologic tests for the detection of serum antibodies to whole-cell and recombinant borrelia burgdorferi antigens in cattle.serum samples from healthy dairy and beef cattle, living in tick-infested areas of connecticut, usa, were analyzed by polyvalent enzyme-linked immunosorbent assays (elisa), indirect fluorescent antibody (ifa) staining methods, or western blot procedures to detect antibodies to tick-borne agents. of the 80 sera tested by elisa with whole-cell or 10 separate recombinant antigens (fusion proteins) of borrelia burgdorferi sensu stricto, 57 (71%) were positive to 1 or more antigens, while 36 (45%) re ...200415368740
infections of granulocytic ehrlichiae and borrelia burgdorferi in white-tailed deer in connecticut.serum or whole blood samples, obtained from 141 white-tailed deer (odocoileus virginianus) in connecticut (usa) during 1980, 1991, and 1996, were analyzed to detect past or current infections of ehrlichia phagocytophila genogroup organisms and borrelia burgdorferi. when the bds or nch-1 strains of granulocytic ehrlichiae were used separately in indirect fluorescent antibody (ifa) staining methods, antibody positivity rates varied from 25 to 64% in 1991 and 1996, respectively. all 50 sera tested ...199910231753
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