| detection of anaplasma phagocytophilum in brazilian dogs by real-time polymerase chain reaction. | anaplasma phagocytophilum was detected in dogs from brazil in the municipalities of seropédica and itaguaí, rio de janeiro state, by real-time polymerase chain reaction (pcr) using sybr green to detect the amplification. of 253 samples, 18 (7.11%) were positive, with a threshold cycle (ct) ranging from 31 to 35 cycles. the pcr product from a positive sample was cloned and sequenced. the sequence obtained demonstrated 100% identity with other a. phagocytophilum sequences published in the genbank ... | 2011 | 21908321 |
| risk factors for anaplasmosis in dairy cows during the peripartum. | anaplasma marginale is endemic in tropical and subtropical areas around the world. some studies have suggested that cows during peripartum may present a transient immunosuppression state and development of clinical signs of anaplasmosis. the aim of this study was to investigate the relationship between some risk factors and the seroprevalence of a. marginale in dairy cows during peripartum in rio de janeiro, brazil. the risk factors analyzed in association with the prevalence of antibodies again ... | 2014 | 24307390 |
| genetic diversity and molecular phylogeny of anaplasma marginale studied longitudinally under natural transmission conditions in rio de janeiro, brazil. | anaplasma marginale is the most prevalent tick-borne pathogen in cattle in tropical and subtropical regions of the world. major surface protein 1a (msp1a) has been found to be a stable genetic marker for identifying a. marginale isolates within geographical regions. it is conserved in cattle during infection and tick-borne transmission of the pathogen. the aim of the present longitudinal study was to determine occurrences of genetic diversity associated with high prevalence of a. marginale under ... | 2015 | 25985719 |
| infection of water buffalo in rio de janeiro brazil with anaplasma marginale strains also reported in cattle. | anaplasma marginale is the most prevalent pathogen of cattle in tropical and subtropical regions of the world and causes the disease bovine anaplasmosis. the importance of water buffalo in the world economy is increasing. in addition, while water buffalo may serve as a reservoir host for a. marginale, the susceptibility of this host for a. marginale cattle strains in brazil has not been reported. the major surface protein 1 alpha (msp1α) gene has been shown to be a stable genetic marker for iden ... | 2014 | 25260335 |
| characterization of two strains of anaplasma marginale isolated from cattle in rio de janeiro, brazil, after propagation in tick cell culture. | ide8 tick cell cultures have been used for the isolation and propagation of several isolates of anaplasma marginale. the genetic heterogeneity of a. marginale strains in cattle is diverse in endemic regions worldwide and the analyses of msp1α (major surface protein 1 alpha) gene sequences have allowed the identification of different strains. this study reports the isolation and propagation of two new isolates of a. marginale in ide8 cells from blood of two cattle and their morphological and mole ... | 2015 | 25468764 |