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prevalence of clostridium botulinum type c in substrates of phosphate-mine settling ponds and implications for epizootics of avian botulism.prevalence and conditions for occurrence of clostridium botulinum type c were examined on phosphate-mine settling ponds and a natural wetland in northern florida between april 1981 and march 1982. substrate samples were collected monthly (winter) and semi-monthly (summer) from 16 locations on seven ponds. selected environmental parameters were measured at each location at the time of sampling. mouse inoculation tests and toxin neutralization tests using enrichment culture filtrates were conducte ...19836358539
hantavirus pulmonary syndrome in florida: association with the newly identified black creek canal virus.hantavirus pulmonary syndrome (hps) is a recently recognized viral zoonosis. the first recognized cases were caused by a newly described hantavirus. sin nombre virus (previously known as muerto canyon virus), isolated from peromyscus maniculatus (deer mouse). we describe a 33-year-old floridian man who resided outside the ecologic range of p maniculatus but was found to have serologic evidence of a hantavirus infection during evaluation of azotemia associated with adult respiratory distress synd ...19968579086
prevalence of antibodies to arenaviruses in rodents from the southern and western united states: evidence for an arenavirus associated with the genus neotoma.the objectives of this study were to extend our knowledge of the geographic distribution and rodent host range of arenaviruses in north america. sera from wild rodents collected from the southern and western united states were tested for antibody against tamiami, pichinde, junin, and lymphocytic choriomeningitis viruses, using an indirect fluorescent antibody test. antibody to at least one arenavirus was found in 220 (3.1%) of 7,106 rodents tested. the antibody-positive animals included mus musc ...19968686773
myocarditis in mice and guinea pigs experimentally infected with a canine-origin borrelia isolate from florida.to characterize the pathogenic potential of a unique borrelia isolate obtained from a dog from florida (fcb isolate).19968712515
genetic reassortment among viruses causing hantavirus pulmonary syndrome.in order to determine the frequency and characteristics of reassortment among viruses causing hantavirus pulmonary syndrome (hps), mixed infections were initiated in tissue culture by using two closely related strains of sin nombre virus, cc107 (from eastern california) and nmr11 (from new mexico), which share the same species of rodent host in nature, the deer mouse (peromyscus maniculatus). potential reassortant virus plaques were screened by multiplex rt-pcr, using primers specific for indivi ...19989501041
adaptive basis of geographic variation: genetic, phenotypic and environmental differences among beach mouse populations.a major goal in evolutionary biology is to understand how and why populations differentiate, both genetically and phenotypically, as they invade a novel habitat. a classical example of adaptation is the pale colour of beach mice, relative to their dark mainland ancestors, which colonized the isolated sandy dunes and barrier islands on florida's gulf coast. however, much less is known about differentiation among the gulf coast beach mice, which comprise five subspecies linearly arrayed on florida ...200919656790
parasitic arthropods of sympatric opossums, cotton rats, and cotton mice from merritt island, florida.six species of parasitic arthropods were collected from 12 opossums (didelphis virginiana), 8 species were obtained from 28 cotton rats (sigmodon hispidus), and 4 species were collected from 10 cotton mice (peromyscus gossypinus) from merritt island, brevard county, florida. the flea polygenis gwyni was the only parasite that infested all 3 host species, and it was present in very high densities (mean intensity = 73.7) on the opossums. the american dog tick, dermacentor variabilis, and the tropi ...19938459341
natural occurrence and characterization of the lyme disease spirochete, borrelia burgdorferi, in cotton rats (sigmodon hispidus) from georgia and florida.this is the first report of natural infection by borrelia burgdorferi in the cotton rat sigmodon hispidus. nine b. burgdorferi isolates were obtained from ear tissues, urinary bladders, or both, by culturing tissues in bskii medium. the rat from which the si-3 isolate was cultured was from the same site (sapelo island, georgia) as an infected cotton mouse peromyscus gossypinus and ixodes scapularis tick reported previously. the 8 b. burgdorferi isolates from rats in florida included 1 (ai-1) fro ...19957876974
chlamydia psittaci infection in companion birds examined in florida.over a two-year period, companion birds, submitted mostly by small-animal practitioners to the animal disease diagnostic laboratory, kissimmee, florida, were examined for chlamydia. chick embryo yolk sac and mouse brain inoculations were used for isolation. chlamydiae were isolated in 58 (20%) of 287 birds examined. although twice as many birds were examined in 1980 as in 1979, the percentage of positive isolations was 20% for each year. this suggests a constant level of chlamydial infection in ...19827092742
biochemical polymorphism and systematics in the genus peromyscus. 3. variation in the florida deer mouse (peromyscus floridanus), a pleistocene relict. 19734705256
an outbreak and review of cave-associated histoplasmosis capsulati.three male college students from florida developed acute onsets of fever, chills, shortness of breath, and cough within one day of each other, and all were eventually hospitalized for four to 29 days. all chest x-ray films showed diffuse reticulonodularities in both lung fields. laboratory studies confirmed the diagnosis of histoplasmosis. the three students had been 'spelunking' (cave exploring) 6 to 7 days before their onset of symptoms. one of four soil samples collected in the caves was posi ...19863746585
mickey mouse neurology. neurological disorders in children visiting central florida.seizures are the most common cause of pediatric neurological admissions in children visiting central florida. in approximately 50%, seizures are brief, generalized and associated with fever. most of the afebrile seizures leading to hospitalization occur in children not previously recognized as having a seizure disorder. sleep-associated seizures ("benign epilepsy of childhood," rolandic seizures) may be recognized more easily during vacations when children often share a hotel room with parents o ...19902370517
comparison of blocking dot elisa and competitive elisa, using a monoclonal antibody for detection of bluetongue virus antibodies in cattle.a blocking (b) dot enzyme-linked immunosorbent assay (elisa), using a monoclonal antibody (mab) against a group specific antigen of bluetongue virus (btv) is described for the detection of btv antibodies to btv in cattle sera. dots of btv antigens were adsorbed to nitrocellulose (nc) strips and/or nc mounted in the windows of dipsticks. after blocking the remaining sites of the nc paper with milk powder solution and immersion in the test sample, the nc strips and dipsticks were exposed to mab. b ...19921319625
field studies on ozone inactivation of a gymnodinium breve toxin.water samples were collected from boca ciega bay (st. petersburg) during the april, 1974, red tide that occurred on the florida west coast. the causative agent of this phenomenon was the toxic dinoflagellate, gymnodinium breve. the toxic red tide samples were treated with ozone gas and rendered nonlethal, as measured by mouse injection.19751204593
residue organic mixtures from drinking water show in vitro mutagenic and transforming activity.indications of possible health effects of residue organics in drinking water have been sought using short-term tests of mutagenic and transforming activity. ten percent or less of the total organic material in drinking water has been identified; the remainder is believed to include thousands of unknown nonvolatile compounds. residual organics were concentrated from drinking water from representative u.s. cities by reverse osmosis followed by liquid-liquid extraction [yielding the reverse osmosis ...1978569710
the selective advantage of crypsis in mice.the light color of mice that inhabit the sandy dunes of florida's coast have served as a textbook example of adaptation for nearly a century, despite the fact that the selective advantage of crypsis has never been directly tested or quantified in nature. using plasticine mouse models of light and dark color, we demonstrate a strong selective advantage for mice that match their local background substrate. further our data suggest that stabilizing selection maintains color matching within a single ...201020163447
the genetic basis of phenotypic convergence in beach mice: similar pigment patterns but different genes.convergent evolution is a widespread phenomenon seen in diverse organisms inhabiting similar selective environments. however, it is unclear if similar phenotypes are produced by the same or different genes and mutations. here we analyze the molecular mechanisms underlying convergent pigment pattern among subspecies of the beach mouse (peromyscus polionotus) inhabiting the gulf and atlantic coasts of florida. in these two geographic regions, separated by more than 300 km, "beach mice" have lighte ...200918832078
natural selection along an environmental gradient: a classic cline in mouse pigmentation.we revisited a classic study of morphological variation in the oldfield mouse (peromyscus polionotus) to estimate the strength of selection acting on pigmentation patterns and to identify the underlying genes. we measured 215 specimens collected by francis sumner in the 1920s from eight populations across a 155-km, environmentally variable transect from the white sands of florida's gulf coast to the dark, loamy soil of southeastern alabama. like sumner, we found significant variation among popul ...200818489719
saxitoxin puffer fish poisoning in the united states, with the first report of pyrodinium bahamense as the putative toxin source.from january 2002 to may 2004, 28 puffer fish poisoning (pfp) cases in florida, new jersey, virginia, and new york were linked to the indian river lagoon (irl) in florida. saxitoxins (stxs) of unknown source were first identified in fillet remnants from a new jersey pfp case in 2002.200617035133
a single amino acid mutation contributes to adaptive beach mouse color pattern.natural populations of beach mice exhibit a characteristic color pattern, relative to their mainland conspecifics, driven by natural selection for crypsis. we identified a derived, charge-changing amino acid mutation in the melanocortin-1 receptor (mc1r) in beach mice, which decreases receptor function. in genetic crosses, allelic variation at mc1r explains 9.8% to 36.4% of the variation in seven pigmentation traits determining color pattern. the derived mc1r allele is present in florida's gulf ...200616825572
borrelia species in host-seeking ticks and small mammals in northern florida.the aim of this study was to improve understanding of several factors related to the ecology and environmental risk of borrelia infection in northern florida. small mammals and host-seeking adult ticks were collected at several sites, and specimens were tested for the presence of borrelia species, primarily by pcr amplification. tissues from some vertebrates and ticks were initially cultured in bsk-h medium to isolate spirochetes, but none were recovered. however, comparison of partial flagellin ...200415528699
fitness effects of a selfish gene (the mus t complex) are revealed in an ecological context.in wild house mice, genes linked to the t transmission distortion complex cause meiotic drive by sabotaging wild-type gametes. the t complex is consequently inherited at frequencies higher than 90%. yet, for unclear reasons, in wild mouse populations this selfish dna is found at frequencies much lower than expected. here, we examine selection on the t complex in 10 seminatural populations of wild mice based on data from 234 founders and nearly 2000 progeny. eight of the 10 populations decreased ...200415266980
ciguatera fish poisoning in the caribbean islands and western atlantic.ciguatera fish poisoning (ciguatera), a common poisoning caused by fish ingestion, is reviewed in the western atlantic and the caribbean waters. it is endemic from florida coasts (northern limit) to martinique island (southern limit), with outbreaks occurring from time to time. in the caribbean, ciguatera causes a polymorphic syndrome with gastrointestinal, cardiovascular, and neurological signs and symptoms. neurological and muscular dysfunctions can be treated by intravenous injection of d-man ...200112882228
staff education at the click of a mouse. 200212132434
genetic heterogeneity of borrelia burgdorferi sensu lato in the southern united states based on restriction fragment length polymorphism and sequence analysis.fifty-six strains of borrelia burgdorferi sensu lato, isolated from ticks and vertebrate animals in missouri, south carolina, georgia, florida, and texas, were identified and characterized by pcr-restriction fragment length polymorphism (rflp) analysis of rrf (5s)-rrl (23s) intergenic spacer amplicons. a total of 241 to 258 bp of intergenic spacers between tandemly duplicated rrf (5s) and rrl (23s) was amplified by pcr. msei and drai restriction fragment polymorphisms were used to analyze these ...200111427560
rodent ectoparasites from two locations in northwestern florida.from feb.-apr. 1999, 19 species of ectoparasitic arthropods (2 sucking lice, 4 fleas, 4 ticks, 2 mesostigmatid mites, 5 chiggers, 2 fur mites) were recovered from 106 rodents belonging to 5 species (cotton mouse, peromyscus gossypinus, n = 64; cotton rat, sigmodon hispidus, n = 23; eastern woodrat, neotoma floridana, n = 9; golden mouse, ochrotomys nuttalli, n = 9; eastern gray squirrel, sciurus carolinensis, n = 1) at tall timbers research station, leon county, florida. during the same period, ...200011217221
patent suit on alzheimer's mouse rejected...but controversy over rights lingers on. 200010890416
celebration health: nothing mickey mouse about this new paradigm. 199810338908
antibodies to granulocytic ehrlichiae in white-footed and cotton mice in eastern united states.serum samples, collected from peromyscus leucopus (white-footed mouse) or peromyscus gossypinus (cotton mouse) during 1987 through 1990 in florida, georgia, maryland, mississippi, and north carolina (usa), and in 1997 in southern connecticut were analyzed by indirect fluorescent antibody (ifa) staining methods or western blot procedures for antibodies to granulocytic ehrlichiae. of the 82 sera from white-footed mice in connecticut tested by ifa methods with either the bds or nch-1 strain of the ...199910231752
theme park ems. it's no mickey mouse operation.in a single shift, they can rescue someone trapped in a time warp, perform cpr while menacing monsters look over their shoulders or calmly watch as a motorboat chase ends in a huge ball of fire. it's just another routine day for ems providers in america's theme parks, where getting ready for unusual calls is the norm. jems went behind the scenes with theme park responders to explore the unique challenges of their service environment. what we found was a group of super-dedicated professionals who ...199510143711
serologic evidence of infection with ehrlichia spp. in wild rodents (muridae: sigmodontinae) in the united states.rodent (muridae: sigmodontinae) blood and sera collected from 14 states were tested for seroreactivity to a cultured isolate of the human granulocytic ehrlichiosis (hge) agent by using an indirect immunofluorescence assay. of the 1,240 samples tested, 136 (11%) were found to be reactive at titers of > or = 32. rodents with hge agent-specific antibodies were found in new york (23% of 491 samples; geometric mean endpoint titer [gmt] = 441), connecticut (11% of 100 samples; gmt = 481), california ( ...19989508298
deactivation mechanisms of rod phototransduction: the cogan lecture.the absorption of photons in rods and cones of the retina activate homologous biochemical signaling cascades that lead to the electrical changes that subserve the first steps in vision. persistent activity of the cascade interferes with the ability of the photoreceptor to signal the absorption of subsequent photons, ultimately limiting the photoreceptor's sensitivity and temporal resolution. this article summarizes recent work on transgenic and knockout mouse rods that has revealed the deactivat ...201020185839
convergence in pigmentation at multiple levels: mutations, genes and function.convergence--the independent evolution of the same trait by two or more taxa--has long been of interest to evolutionary biologists, but only recently has the molecular basis of phenotypic convergence been identified. here, we highlight studies of rapid evolution of cryptic coloration in vertebrates to demonstrate that phenotypic convergence can occur at multiple levels: mutations, genes and gene function. we first show that different genes can be responsible for convergent phenotypes even among ...201020643733
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