| mice transgenic for a human porcine endogenous retrovirus receptor are susceptible to productive viral infection. | porcine endogenous retrovirus (perv) is considered one of the major risks in xenotransplantation. no valid animal model has been established to evaluate the risks associated with perv transmission to human patients by pig tissue xenotransplantation or to study the potential pathogenesis associated with perv infection. in previous work we isolated two genes encoding functional human perv receptors and proved that introduction of these into mouse fibroblasts allowed the normally nonpermissive mous ... | 2006 | 16537582 |
| functional hierarchy of two l domains in porcine endogenous retrovirus (perv) that influence release and infectivity. | the porcine endogenous retrovirus (perv) gag protein contains two late (l) domain motifs, pppy and p(f/s)ap. using viral release assays we demonstrate that pppy is the dominant l domain involved in perv release. pfap represents a novel retroviral l domain variant and is defined by abnormal viral assembly phenotypes visualized by electron microscopy and attenuation of early perv release as measured by viral genomes. psap is functionally dominant over pfap in early perv release. psap virions are 3 ... | 2008 | 18355887 |
| exceptional diversity of mouse lemurs (microcebus spp.) in the makira region with the description of one new species. | although the number of described lemur species has increased considerably over the last 20 years, detailed biogeographic data are still lacking from many geographic regions, in particular in the eastern part of madagascar. this study investigated mouse lemur species diversity in a previously unstudied inter-river-system in the eastern makira region. three sites were visited and 26 individuals were sampled and characterized with 13 external morphometric measurements. standard phylogenetic analyse ... | 2008 | 18626970 |
| anti-influenza virus activity of tricin, 4',5,7-trihydroxy-3',5'-dimethoxyflavone. | we examined the anti-influenza virus activity of tricin, 4',5,7-trihydroxy-3',5'-dimethoxyflavone, against five viruses: a/solomon islands/3/2006 (h1n1), a/hiroshima/52/2005 (h3n2), a/california/07/2009 (h1n1pdm), a/narita/1/2009 (h1n1pdm) and b/malaysia/2506/2004 strains in vitro and against a/pr/8/34 virus in vivo. | 2011 | 21860068 |
| stimulation of fructose 1,6-bisphosphate production in melanoma cells by alpha-melanocyte-stimulating hormone-- 31p/13c-nmr and 32p-labeling studies. | in a 31p-nmr spectroscopic study of cultured m2r mouse melanoma cells, we previously demonstrated the acute stimulation of three peaks in the phosphomonoester region of the spectrum by [ahx4, dphe7]alpha-melanotropin (concomitant with an increase in cellular adenosine 3',5'-phosphate (camp) and a decrease in atp [degani, h., dejordy, j. o. & salomon, y. (1991) proc. natl acad. sci. usa 88, 1506-1510]. chemical identification of these metabolites was performed in this study using 32p metabolic la ... | 1998 | 9851693 |
| detection of nearest neighbors to specific fluorescently tagged ligands in rod outer segment and lymphocyte plasma membranes by photosensitization of 5-iodonaphthyl 1-azide. | lima bean agglutinin-fluorescein 5-isothiocyanate conjugate (fluncs-lima bean lectin) interacts with specific receptor molecules on membranes both from the rod outer segment (ros) of the frog retina and from s49 mouse lymphoma cells. when [125i]-5-iodonaphthyl 1-azide (125i-ina), which freely and randomly partitions into the lipid bilayer, is added to membranes and the suspension is irradiated at 480 nm, the fluncs-conjugated lectin photosensitizes the [125i]ina but only at discrete sites. this ... | 1989 | 2496754 |
| 8-chloro-camp inhibits transforming growth factor alpha transformation of mammary epithelial cells by restoration of the normal mrna patterns for camp-dependent protein kinase regulatory subunit isoforms which show disruption upon transformation. | differential regulation of the regulatory subunits of camp-dependent protein kinase isozymes correlates with the growth inhibitory effect of site-selective 8-cl-camp demonstrated in cancer cell lines (ally, s., tortora, g., clair, t., grieco, d., merlo, g., katsaros, d., ogreid, d., døskeland, s.o., jahnsen, t., and cho-chung, y.s. (1988) proc. natl. acad. sci. u. s. a. 85, 6319-6322). such selective modulation of protein kinase isozyme regulatory subunits was also found in the 8-cl-camp-induced ... | 1990 | 1688548 |
| monoclonal antibodies to the haemagglutinin ha1 subunit of the pandemic influenza a/h1n1 2009 virus and potential application to serodiagnosis. | in order to provide specific serological reagents for pandemic influenza a/h1n1 2009 virus, monoclonal antibodies (mabs) to recombinant haemagglutinin component ha1 (rha1) were generated after fusing spleen cells from a mouse immunized with rha1 protein derived from influenza strain a/california/06/09 h1n1 with a mouse myeloma cell line. five hybridoma clones secreting mabs specific for the rha1 protein derived from pandemic influenza a/h1n1 2009 and not for rha1 from seasonal h1n1 influenza str ... | 2011 | 21328368 |