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virulence in mice of orientia tsutsugamushi isolated from patients in a new endemic area in japan.four strains of orientia tsutsugamushi (kn-1, kn-2, kn-3 and gj-1) isolated from patients in an area of gifu prefecture, japan, in which tsutsugamushi disease is newly endemic, were examined for their virulence in mice. among these, kn-1 (identified as kawasaki type), gj-1 (identified as kuroki type) and kn-2 strains were found to be non-lethal for balb/c mice as well as ch3/hej mice, even with high doses (10(6) x being the 50% mouse infectious dose). on the other hand, the kn-3 strain was found ...19968981347
comparison of ehrlichia muris strains isolated from wild mice and ticks and serologic survey of humans and animals with e. muris as antigen.in metropolitan tokyo, the ehrlichia muris seropositivity rate of 24 wild mice was 63% in hinohara village, but in the surrounding areas, it was 0 to 5%. this finding suggests that the reservoir of e. muris is focal. among the 15 seropositive mice, ehrlichiae were isolated from 9 apodemus speciosus mice and 1 a. argenteus mouse, respectively. five ehrlichial isolates were obtained from 10 ticks (haemaphysalis flava) collected in asuke town, aichi prefecture, where the e. muris type strain had be ...199910074536
genetic and antigenic characterization of newly isolated bovine toroviruses from japanese cattle.torovirus, a member of the coronaviridae family, is a gastrointestinal infectious agent that has been identified in humans, cattle, pigs, and equines. toroviruses, except equine torovirus, are difficult to propagate in cell culture; indeed, to date, only the aichi/2004 strain of bovine torovirus (btov) has been isolated among the human, bovine, and porcine toroviruses. in the present study, four cytopathogenic btovs were isolated from diarrheal feces of the cattle using the hrt-18 cell line, and ...201020220164
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