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surveillance of the mosquito aedes aegypti and its biocontrol with the copepod mesocyclops aspericornis in australian wells and gold mines.a survey of the dengue vector mosquito aedes aegypti was undertaken using funnel traps to detect immature stages (larvae and pupae) in flooded disused mine shafts and wells in charters towers, queensland, northern australia. the town has a history of dengue fever since 1885 when goldminers were the first recorded victims. during the latest dengue epidemic in 1993, 2% of the population had laboratory-confirmed dengue virus type 2, despite source reduction of ae.aegypti breeding-sites at ground le ...19968744708
determinants of dengue 2 infection among residents of charters towers, queensland, australia.dengue fever is caused by one of the four serotypes of the dengue virus and is transmitted by the urban mosquito aedes aegypti. in 1993, the city of charters towers in the tropical north of australia experienced an epidemic caused by the dengue 2 virus. a cross-sectional sample of 1,000 people was assessed for determinants of recent symptomatic dengue infection. after exclusion of people with prior exposure to dengue 2, a study group of 797 persons, including 196 patients with recent infection, ...19989850134
vector competence of aedes notoscriptus (diptera: culicidae) for barmah forest virus and of this species and aedes aegypti (diptera: culicidae) for dengue 1-4 viruses in queensland, australia.aedes notoscriptus (skuse) mosquitoes colonized from brisbane, queensland, australia, were fed on blood suspensions containing either barmah forest (bf) virus, dengue virus type 1 (den-1), dengue virus type 2 (den-2), dengue virus type 3 (den-3), or dengue virus type 4 (den-4). ae. notoscriptus was shown to be moderately susceptible to oral infection with bf virus, id50 = 10(3.3) ccid50 per mosquito, with titers in infected mosquitoes peaking 10 d after infection. bf virus transmission occurred ...199910467781
enhanced vector competence of aedes aegypti (diptera: culicidae) from the torres strait compared with mainland australia for dengue 2 and 4 viruses.australian aedes aegypti (l.) mosquitoes colonized from the torres strait and three mainland localities (charters towers, townsville, and cairns) were fed on blood suspensions containing dengue virus type 2 (den-2) or dengue virus type 4 (den-4). variation was found in oral susceptibility to den-2 (59 -99% infection) and den-4 (28-79% infection) among ae. aegypti assayed for virus at 8, 12, 16, or 20 d after ingestion of infected blood. torres strait ae. aegypti were the most susceptible to den- ...200314765675
infection and dissemination of dengue virus type 2 in aedes aegypti, aedes albopictus, and aedes scutellaris from the torres strait, australia.to determine their relative roles in transmission of dengue virus (denv) in the torres strait region of northern australia, we examined infection and dissemination of a sympatric strain of dengue virus type 2 (denv-2) in aedes scutellaris, ae. albopictus, and ae. aegypti. in experiments using membrane feeders for virus exposure, infection rates were 83% and 43% for ae. scutellaris and ae. aegypti, respectively. salivary gland infection rates for both species were 43%. in experiments using pledge ...200718240514
the 1993 dengue 2 epidemic in north queensland: a serosurvey and comparison of hemagglutination inhibition with an elisa.an epidemic of dengue type 2 infection occurred in north queensland during 1992 and 1993. a random serosurvey of 1,000 residents of a population that experienced this epidemic only during 1993 was conducted to determine the proportion of the population at risk for secondary infection in the event of another epidemic with a different serotype. the ability of an elisa to detect prior exposure to the dengue virus was compared with the hemagglutination inhibition assay. dengue 2 virus plaque-reducti ...19989749644
the 1993 dengue 2 epidemic in charters towers, north queensland: clinical features and public health impact.in 1993 an epidemic caused by dengue virus type 2 occurred in several north queensland population centres. charters towers, estimated population 10,000, had 155 officially notified cases. an analysis of symptoms was undertaken using a random sample of 1000 residents to determine specificity of symptoms, the subclinical infection rate, and to establish the true extent of the epidemic. retrospective diagnoses of dengue fever were based on the presence of both serum dengue 2 neutralizing antibody a ...19989747766
quantifying the spatial dimension of dengue virus epidemic spread within a tropical urban environment.dengue infection spread in naive populations occurs in an explosive and widespread fashion primarily due to the absence of population herd immunity, the population dynamics and dispersal of ae. aegypti, and the movement of individuals within the urban space. knowledge on the relative contribution of such factors to the spatial dimension of dengue virus spread has been limited. in the present study we analyzed the spatio-temporal pattern of a large dengue virus-2 (denv-2) outbreak that affected t ...201021200419
development of an antigen capture immunoassay based on monoclonal antibodies specific for dengue virus serotype 2 nonstructural protein 1 for early and rapid identification of dengue virus serotype 2 infections.the dengue virus (denv) has four distinct serotypes (denv1, denv2, denv3, and denv4) that require differentiation for effective prevention of morbid diseases. the recently developed denv1-specific ns1 antigen capture enzyme-linked immunosorbent assay (elisa) based on the monoclonal antibodies (mabs) that recognize distinct epitopes on nonstructural protein 1 (ns1) of a specific denv serotype is convenient and cost-effective, but assays have not yet been developed for denv serotypes 2 to 4. this ...200919020106
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