| acidic β-mannanase from penicillium pinophilum c1: cloning, characterization and assessment of its potential for animal feed application. | the β-mannanase gene, man5c1, was cloned from penicillium pinophilum c1, a strain isolated from the acidic wastewater of a tin mine in yunnan, china, and expressed in pichia pastoris. the sequence analysis displayed the gene consists of a 1221-bp open reading frame encoding a protein of 406 amino acids (man5c1). the deduced amino acid sequence of man5c1 showed the highest homology of 57.8% (identity) with a characterized β-mannanase from aspergillus aculeatus belonging to glycoside hydrolase fam ... | 2011 | 22036533 |