microsporidiosis in south africa: pcr detection in stool samples of hiv-positive and hiv-negative individuals and school children in vhembe district, limpopo province.microsporidia were initially recognized as pathogens of insects and fish but have recently emerged as an important group of human pathogens, especially in immune-compromised individuals, such as those with hiv infection. in this study, we used a pcr-rflp assay confirmed by quantitative real-time pcr and trichrome staining to determine the prevalence of microsporidian infections among hospital patients and school children in vhembe region. enterocytozoon bieneusi was the only microsporidian speci ...200717412378
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