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human infection with swine influenza virus--wisconsin. 19882846999
swine artificial insemination in wisconsin. 19645217514
hong kong influenza infection in swine: experimental and field observations.the recovery of influenzaviruses indistinguishable from a/hong kong/68 virus from pigs in taiwan in 1970 provided the first direct evidence of the interspecies transfer of influenzaviruses. pigs were infected experimentally with that virus and transmitted the virus to pen mates. there was no evidence of a/hong kong/68 antibodies in serum collected from pigs prior to 1968. such antibodies were detected in serum collected from pigs that lived during the period when hong kong influenza was epidemic ...19724540999
swine influenza: epizootiological and serological studies.studies of naturally occurring respiratory diseases in the midwestern parts of the usa showed that swine influenza is still prevalent and that mild forms as well as the classical forms of swine influenza occur. outbreaks of respiratory disease of unknown etiology that are clinically similar to swine influenza were also found. on some farms, swine influenza occurred first in farrowing pens. it did not occur on some farms where the disease had occurred in previous years. this disappearance may hav ...19724540998
la crosse virus isolations from mosquitoes in wisconsin, 1964-68. 19724399845
california serogroup virus infections in wisconsin domestic animals.a serologic survey and experimental virus transmission studies were done to assess the role of domestic animals as amplifier hosts of la crosse (lacv) and jamestown canyon (jcv) viruses. serum from 319 cows, 88 dogs, 122 equines, 47 swine, 10 goats, and 4 cats were tested for neutralizing antibody to lacv, jcv, trivittatus (tvtv), and snowshoe hare (sshv) viruses. antibody prevalences of lacv, tvtv, and sshv were less than 10% in all species. antibody to jcv was detected in all species except ca ...19883189702
leads from the mmwr. human infection with swine influenza virus--wisconsin. 19882846902
antigenic conservation of h1n1 swine influenza viruses.influenza viruses of the h1n1 subtype have been continually circulating in pigs in the u.s.a. for at least 50 years. to examine the level of antigenic variation in these swine viruses, a panel of 60 monoclonal antibodies (mabs) to the haemagglutinin (ha) of recent swine isolates was prepared. evaluation of neutralization escape mutants selected with these mabs defined four antigenic sites on the ha, two of which overlap. swine viruses isolated over 24 years in an enzootic area in wisconsin were ...19892558159
fatal swine influenza pneumonia during late pregnancy.serious morbidity or death from swine influenza infection is unusual in the immunocompetent host. we present a fatal case of pneumonia caused by this virus in a previously healthy 32-year-old woman during her third trimester of pregnancy, and review all published case reports of swine influenza in united states civilians. pregnancy may be a predisposing factor to fulminant infection with swine influenza virus.19902153372
swine influenza virus infections. transmission from ill pigs to humans at a wisconsin agricultural fair and subsequent probable person-to-person transmission.in september 1988, a previously healthy 32-year-old pregnant woman was hospitalized for pneumonia and died 8 days later. the only pathogen detected was an influenza virus antigenically related to the swine influenza virus (siv). four days before illness onset, the patient visited a county fair swine exhibition where there was widespread influenzalike illness among the swine. to detect other persons who were possibly infected by contact with the ill swine, we measured serum siv hemagglutination-i ...19911845913
swine influenza surveillance, wisconsin agricultural fairs, 1989 and 1990. 19921315876
swine-like influenza virus infection in a wisconsin farm family. 1977564374
swine influenza virus infections in humans.influenza in swine was first recognized as an epizootic disease in 1918. during that same year influenza virus in humans caused the worst pandemic on record. the virus of swine influenza was isolated in 1930. swine influenza virus was first isolated from humans in 1974. since then, including the cases at fort dix, there have been a total of nine viral isolations from humans in the united states. serologic evidence of infections with swine influenza virus in humans has also been obtained. evidenc ...1977342616
the severity of pandemic h1n1 influenza in the united states, from april to july 2009: a bayesian analysis.accurate measures of the severity of pandemic (h1n1) 2009 influenza (ph1n1) are needed to assess the likely impact of an anticipated resurgence in the autumn in the northern hemisphere. severity has been difficult to measure because jurisdictions with large numbers of deaths and other severe outcomes have had too many cases to assess the total number with confidence. also, detection of severe cases may be more likely, resulting in overestimation of the severity of an average case. we sought to e ...200919997612
prevalence and antimicrobial resistance profile of campylobacter spp. isolated from conventional and antimicrobial-free swine production systems from different u.s. regions.we conducted a study to compare the prevalence and antimicrobial resistance profile of campylobacter isolated from 34 farm-slaughter pair cohorts of pigs raised in conventional and antimicrobial-free (abf) production systems. isolates originated from four different states of two geographic regions (region 1--ohio and michigan; region 2--wisconsin and iowa). a total of 838 fecal and 1173 carcass samples were examined. campylobacter isolates were speciated using multiplex polymerase chain reaction ...201021133777
influenza surveillance of wisconsin (usa) population-detection of a/new jersey by isolation and serologic monitoring and vaccine evaluation.the 1976-77 influenza surveillance in wisconsin consisted of three major areas of study. serum and virus isolation specimens were obtained from the practicing medical community and from epidemiologic studies. from all sources 1,132 throat specimens were tested by egg and tissue culture inoculations. three isolations similar to a/new jersey/8/76 were recovered from pig farm associated patients. one contact with one of the isolate patients seroconverted to a/new jersey suggesting patient to patien ...1977342315
natural incidence of stomach worms in domestic swine from georgia & wisconsin. 1977244163
antigenic comparison of swine influenza virus isolates.cross hemagglutination inhibition (hi) and neuraminidase inhibition (ni) tests demonstrated strong antigenic relationship between a/swine/wisconsin/1/73 (sw/73) and a/swine/shope/15/31 (sw/31) influenza viruses. an eigthyone fold purification of virus was achieved by adsorption and elution followed by differential ultracentrifugation and sedimentation through linear sucrose gradient. radioimmunoassay using purified 125i labeled viral antigens revealed antigenic variation between the two virus is ...1975241310
assessment of the hazards of polybrominated biphenyls.during their peak use period, pbbs represented under 1% of the total sales of fire retardant chemicals, and very probably would have escaped intensive study if they had not been mixed accidentally with animal feed preparations. instead, international attention was drawn to pbbs by the state-supervised killing of over 35,000 cattle which had been contaminated with pbbs. interestingly, low doses of pbbs exert a broad spectrum of toxicological, pharmacological, and biochemical effects despite low a ...1978209999
health problems in veterinary students after visiting a commercial swine farm.in october 1993 and 1994, respectively, 77 and 76 third-year veterinary students visited a swine farm to work with pigs for 3 h. on both occasions, a large number of students reported flu-like symptoms after the visit. to further investigate this, the students were presented with a questionnaire modeled after the standard questionnaire used for evaluating organic dust exposure. general and/or respiratory symptoms were reported by 103/142 (72.5%) students. general symptoms, such as eye irritation ...19989442939
wisconsin's "stale calf" issue and a study designed to resolve some of the animal welfare concerns.a unique law (act 201) requiring livestock markets to place an identifying mark on calves up to 90 kg each time they are sold went into effect in wisconsin in 1993. the intent of the law is to reduce the number of times calves are resold and hence become "stale." the original proponents of the law proposed that calves be ear-notched each time they are sold. ear notching, however, was resisted by wisconsin regulatory agencies partly because of fear of an adverse public reaction. these authors the ...19948002445
airborne contaminants and farmers health in swine farms with high and low prevalence of respiratory diseases in pigs.the main purpose of this study was to investigate the relationship between prevalence of respiratory disease in swine and respiratory health of swine farmers. fourteen farms were selected based on clinical history and slaughtercheck evidence of respiratory problems in pigs. the farms were divided into two groups with either high (n = 7) or low (n = 7) prevalence of respiratory disease in pigs. airborne dust, endotoxin and peptidoglycan were measured once in farrowing, gestation, nursery and fini ...19989852495
current studies on the mastitis-metritis-agalactia complex of swine in wisconsin. 19695392152
leptospirosis serology in wisconsin cattle and swine. 19675257310
gastric ulcers in swine under modern intensified husbandry. 19715209513
prevalence of internal parasites in wisconsin specific-pathogen-free swine. 19664958756
heterogeneity and seroprevalence of a newly identified avian hepatitis e virus from chickens in the united states.we recently identified and characterized a novel virus, designated avian hepatitis e virus (avian hev), from chickens with hepatitis-splenomegaly syndrome (hs syndrome) in the united states. avian hev is genetically related to but distinct from human and swine hevs. to determine the extent of genetic variation and the seroprevalence of avian hev infection in chicken flocks, we genetically identified and characterized 11 additional avian hev isolates from chickens with hs syndrome and assessed th ...200212409397
survey of the prevalence of gastric ulcers in swine. 196414227169
iodixanol density gradient preparation in university of wisconsin solution for porcine islet purification.generally, prior to the purification of isolated pancreatic islets, the collagenase-digested tissue is incubated in the university of wisconsin solution (uws; approximately 320 mosm) for osmotic stabilization to preserve or improve the density differences between islets and acinar fragments. the adverse effects arising from the subsequent pelleting and resuspension of the islets in a second, different (often highly hyperosmotic) purification solution are avoided in the protocol described here; p ...200314646009
death by hyperventilation: a common and life-threatening problem during cardiopulmonary resuscitation.this translational research initiative focused on the physiology of cardiopulmonary resuscitation (cpr) initiated by a clinical observation of consistent hyperventilation by professional rescuers in out-of-hospital cardiac arrest. this observation generated scientific hypotheses that could only ethically be tested in the animal laboratory.200415508657
human case of swine influenza a (h1n1) triple reassortant virus infection, wisconsin.zoonotic infections with swine influenza a viruses are reported sporadically. triple reassortant swine influenza viruses have been isolated from pigs in the united states since 1998. we report a human case of upper respiratory illness associated with swine influenza a (h1n1) triple reassortant virus infection that occurred during 2005 following exposure to freshly killed pigs.200818760023
factors associated with salmonella prevalence on pork carcasses in very small abattoirs in wisconsin.the u.s. department of agriculture has expressed concern over salmonella prevalence on pork carcasses. our objectives were to survey the prevalence of salmonella on pork carcasses in very small wisconsin abattoirs, and identify processing conditions and indicator bacteria levels associated with reduced salmonella prevalence. during april to july 2007, sponge samples were obtained from 181 pork carcasses at 10 wisconsin abattoirs before carcass washing (carcass half a), and after washing and chil ...200919435217
rapid multiplex reverse transcription-pcr typing of influenza a and b virus, and subtyping of influenza a virus into h1, 2, 3, 5, 7, 9, n1 (human), n1 (animal), n2, and n7, including typing of novel swine origin influenza a (h1n1) virus, during the 2009 outbreak in milwaukee, wisconsin.a large outbreak of novel influenza a (h1n1) virus (swine origin influenza virus [s-oiv]) infection in milwaukee, wi, occurred in late april 2009. we had recently developed a rapid multiplex reverse transcription-pcr enzyme hybridization assay (fluplex) to determine the type (a or b) and subtype (h1, h2, h3, h5, h7, h9, n1 [human], n1 [animal], n2, or n7) of influenza viruses, and this assay was used to confirm the diagnoses for the first infected patients in the state. the analytical sensitivit ...200919641063
rapid semiautomated subtyping of influenza virus species during the 2009 swine origin influenza a h1n1 virus epidemic in milwaukee, wisconsin.in the spring of 2009, a novel influenza a (h1n1) virus (swine origin influenza virus [s-oiv]) emerged and began causing a large outbreak of illness in milwaukee, wi. our group at the midwest respiratory virus program laboratory developed a semiautomated real-time multiplex reverse transcription-pcr assay (seasonal), employing the nuclisens easymag system (biomérieux, durham, nc) and a raider thermocycler (handylab inc., ann arbor, mi), that typed influenza a virus, influenza b virus, and respir ...200919641066
seroprevalence of and risk factors for toxoplasma gondii in the us swine herd using sera collected during the national animal health monitoring survey (swine 2006).the united states department of agriculture (usda) initiated the national animal health monitoring system (nahms) in 1983 to collect, analyse and disseminate data on animal health, management and productivity in us domestic livestock populations, including swine. the programme includes an on-farm serological sampling component which can be used to monitor seroprevalence of various pathogens, including toxoplasma gondii. the purpose of this study was to determine the seroprevalence of t. gondii i ...201019744304
development of a rapid automated influenza a, influenza b, and respiratory syncytial virus a/b multiplex real-time rt-pcr assay and its use during the 2009 h1n1 swine-origin influenza virus epidemic in milwaukee, wisconsin.rapid, semiautomated, and fully automated multiplex real-time rt-pcr assays were developed and validated for the detection of influenza (flu) a, flu b, and respiratory syncytial virus (rsv) from nasopharyngeal specimens. the assays can detect human h1n1, h3n2, and swine-origin (s-oiv) h1n1 flu a viruses and were effectively used to distinguish flu a infections (of all subtypes) from flu b and rsv infections during the current s-oiv outbreak in milwaukee, wi. the analytical limits of detection we ...201019959800
stomach ulcers in farrowing gilts. 19665947998
outbreak of influenza a/ussr/77 at marquette university.an outbreak of influenzalike illness occurred at marquette university, milwaukee, wisconsin, in february 1978. epidemiologic and laboratory data indicate that the outbreak was due to influenza a/ussr/77 (n1h1) virus. a self-administered questionnaire inquiring about the presence and spectrum of illness was given to faculty members, dental students, and residents of an undergraduate dormitory. age-specific attack rates were 61.5% for persons less than or equal to 22 years of age, 24.7% for those ...19807441004
risk of hypoglycemia in users of human insulin. the wisconsin epidemiologic study of diabetic retinopathy.to evaluate whether there has been a change in frequency of hypoglycemic reactions associated with use of insulin of animal or human type.19979051383
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