| detection of lawsonia intracellularis in faeces of swine from the main producing regions in brazil. | swine proliferative enteropathy is an enteric disease caused by lawsonia intracellularis which affects animals between 6 and 20 weeks of age, causing diarrhea, anorexia, and poor growth. the presence of l. intracellularis was evaluated in the faecal samples of 636 swine from 75 randomly chosen herds in the main swine-producing regions of brazil. the pathogen was detected by the polymerase chain reaction method (pcr) using l. intracellularis specific primers. a 319-bp dna fragment specific for l. ... | 1999 | 10408095 |
| [occurrence of anti-toxoplasma gondii antibodies in swine and ovine slaughtered at municipality of guarapuava in the state of paraná, brazil]. | this study evaluated sero-occurrence of toxoplasmosis in swine and ovine slaughtered in the municipality of guarapuava in the state of paraná. blood samples from 117 swine and 157 ovine were collected. indirect immunofluorescence assay (ifa) was used to detect igg anti-toxoplasma gondii antibodies. serum samples were considered positive at dilutions >or=1:64. out of 117 swine blood sera, 10 (8.54%) were positive, while 11 out of 157 ovine samples (7.0%) showed a reaction to t. gondii antibodies. ... | 2007 | 17588324 |
| high genetic diversity in rdrp gene of brazilian porcine sapovirus strains. | sapovirus is one genus within caliciviridae family that causes diarrhea in humans and animals. sapovirus (sav) has been classified into seven genogroups (gi to gvii). the giii, gvi, and gvii, which prototype is cowden, jj681, and k7/jp strains, respectively, infect pigs. the objective of this study was to characterize wild-type brazilian sav strains from piglet stool samples and determine sav infection frequency, age distribution and association with diarrheic disease. stool samples from 113 pig ... | 2008 | 18403136 |
| prevalence and risk factors for toxoplasma gondii infection in certified and non-certified pig breeding farms in the toledo microregion, pr, brazil. | toxoplasma gondii infection has been diagnosed in pigs all over the world. economical losses are generally related to reproductive disorders. toxoplasma infection is also a matter of public health because tissue cysts of the parasite may remain in pork and pork products, and become sources of human infection. the objective of this study was to evaluate the frequency and risk factors associated with toxoplasma infection in certified and non-certified pig breeding farms in the toledo microregion, ... | 2010 | 20943018 |
| new isolation of leishmania enriettii muniz and medina, 1948 in paranástate, brazil, 50 years after the first description, and isoenzymatic polymorphism of the l. enriettii taxon. | three cases of cutaneous leishmaniasis in guinea-pigs from a rural area near curitiba (paraná state, brazil) are reported. the three parasite isolates were characterized by isoenzyme electrophoresis as leishmania enriettii, of which two distinct zymodemes were observed. | 1996 | 8915125 |
| toxoplasma gondii in fresh pork sausage and seroprevalence in butchers from factories in londrina, paraná state, brazil. | the aims of this study were to verify the presence of toxoplasma gondii cysts in fresh pork sausage and the presence of antibodies against t. gondii in serum of workers from factories with municipal inspection service, in londrina, pr, brazil. 149 samples of sausage were collected from eight factories and blood samples from 47 workers. we also took information about the practices that were adopted in the factories and the workers' habits that could influence the prevalence of toxoplasmosis. afte ... | 2005 | 16138196 |
| prevalence and virulence properties of vibrio cholerae non-o1, aeromonas spp. and plesiomonas shigelloides isolated from cambé stream (state of paraná, brazil). | the incidence of vibrio cholerae, aeromonas spp. and plesiomonas shigelloides was determined in water samples from cambé stream. the samples were collected from seven different sites. the serogroups, virulence markers and drug resistance profiles were also evaluated. twelve aer. hydrophila, 12aer. caviae, eight aer. sobria, seven ple. shigelloides and two v. cholerae non-o1 were isolated. they belonged to different serogroups and all produced haemolysis in different assays. five of the aeromonas ... | 2000 | 10945781 |
| [cholesterol in beef, pork, chicken and their products commercialized in maringá, paraná, brazil]. | a rapid method developed by al-hasani et al. with modifications for cholesterol determination was applied in samples of beef, pork, chicken and meat products. analyses of cholesterol were performed by capillary gas chromatography. the obtained results showed that chicken presented higher concentration (126.96 to 188.29 mg/100 g) of cholesterol in comparison to beef and pork and similar to meat products, except bovine liver that presented 265.03 mg/100 g. cholesterol values of beef and pork sampl ... | 1997 | 9673687 |
| [diversity, distribution and abundance of sandflies (diptera: psychodidae) in parana state, southern brazil]. | due to the spread of visceral leishmaniasis in brazilian territory, this research was undertaken with the objective to identify the sandfly fauna and aspects of the species behavior in forest and anthropic environments in paraná state, brazil. the sandfly captures were sporadically carried out in 37 municipalities of the paraná state, during the period from march 2004 to november 2005. each municipality was once visited, when falcão traps were installed during three consecutive nights in foreste ... | 2008 | 18506303 |
| [preliminary study on feeding preference of nyssomyia neivai (pinto) and nyssomyia whitmani (antunes & coutinho) (diptera: psychodidae) in a rural area of the state of paraná, south brazil]. | due to the importance of sandflies in the american cutaneous leishmaniasis epidemiology, we investigated the feeding preference of sandflies related to domestic animals common in domiciliary environments in endemic rural areas, where this disease occurs. the sandflies were collected with falcão light traps, installed in cages, each one containing one domestic animal kind (pig, dog, rabbit or chicken), in recanto marista, in doutor camargo municipality. forty five collections were made, totaling ... | 2007 | 18246272 |
| g and p genotypes of group a rotavirus strains circulating in calves in brazil, 1996-1999. | fifty fecal samples from calves with diarrhea, positive for group a rotavirus by enzyme immunoassay (eia) and polyacrylamide gel electrophoresis (page) were analyzed by a nested multiplex reverse transcription/polymerase chain reaction (nested multiplex/rt-pcr) for identification of p and g genotypes. samples were collected between 1996 and 1999 from eight dairy and/or beef cattle herds located in the mato grosso do sul, são paulo, goiás and paraná states, brazil. complete genotyping was possibl ... | 2004 | 15066719 |
| absence of trichinella infection in adult pigs slaughtered in palmas, state of parana (brazil), detected by modified artificial digestion assay. | samples of 2,490 adult pigs, slaughtered under federal inspection between may 2004 and february 2005 in the county of palmas, state of paraná, brazil, were examined by pooled sample artificial digestion with magnetic stirrer assay for a survey of trichinella spp. larvae. animals originated from 53 counties in three states of southern brazil. test sensitivity was increased with modifications of the european standard for artificial digestion. in this survey, a 5-g sample of tongue and 5-g sample o ... | 2006 | 16541706 |