denaturing gradient gel electrophoresis analysis of the 16s rrna gene v1 region to monitor dynamic changes in the bacterial population during fermentation of italian sausages. | in this study, a pcr-denaturing gradient gel electrophoresis (dgge) protocol was used to monitor the dynamic changes in the microbial population during ripening of natural fermented sausages. the method was first optimized by using control strains from international collections, and a natural sausage fermentation was studied by pcr-dgge and traditional methods. total microbial dna and rna were extracted directly from the sausages and subjected to pcr and reverse transcription-pcr, and the amplic ... | 2001 | 11679334 |