| restriction fragment length polymorphism (rflp) analysis of genomic dna of 5 strains of trichinella spiralis in china. | five restriction endonucleases were used to digest genomic dna from 5 isolates of trichinella spiralis obtained from changchun, tianjin, xian, henan and yunnan. all the isolates were secured from pigs except the changchun strain which came from dog. the dna fragments digested by endonuclease were separated by agarose gel electrophoresis. the changchun isolate had a ecori band at 1.12kb and a drai band at 1.97kb which were unique to this isolate. a cloned specific repetitive dna sequence (1.12kb) ... | 1995 | 8580480 |
| analysis of chinese isolates of trichinella spiralis by molecular biotechnology. | to investigate the relationships among trichinella spiralis isolates obtained from different hosts and geographical regions in china. | 1996 | 9275332 |
| pcr-sscp of rdna for the identification of trichinella isolates from mainland china. | a polymerase chain reaction (pcr)-based single strand conformation polymorphism (sscp) analysis of the expansion segment 5 (domain iv) of the large subunit of ribosomal dna was employed to characterize seven isolates of trichinella from china (a-g), including six of pig origin from regions in dengxian (a), tianjin (b), harbin (d), baoshan (e), xinye (f) and xian (g), and one of canine origin from changchun (c). isolates a, d, e, f and g were classified as trichinella spiralis based on sscp patte ... | 1998 | 9584075 |
| outbreaks of human trichinellosis caused by consumption of dog meat in china. | dog meat has become an important source of trichinella infection for humans in china. the first documented outbreak of human trichinellosis resulting from the consumption of dog meat occurred in china in 1974. until 1999, the outbreaks with this source of infection occurred mostly in northeast of china (81 cases in five outbreaks in jilin and two in liaoning), beijing (six cases) and henan provinces (two cases). the epidemiological surveys were performed in nine provinces or autonomous regions o ... | 2001 | 11484389 |
| seroepidemiology and genetic characterization of hepatitis e virus in the northeast of china. | the aim of this study was to analyze the prevalence of infection and genotype of hepatitis e virus (hev) in people and animals in the northeast of china (heilongjiang, jilin and liaoning provinces). this seroepidemiological study was conducted using enzyme immunoassays and human sera positive for hev antigen or anti-hev igm, and animal sera positive for hev antigen or with an s/co <or=10 for anti-hev were tested for hev rna using real-time rt-pcr and nested rt-pcr. in humans, the overall prevale ... | 2009 | 19460321 |
| vomiting and wasting disease associated with hemagglutinating encephalomyelitis viruses infection in piglets in jilin, china. | one coronavirus strain was isolated from brain tissues of ten piglets with evident clinical manifestations of vomiting, diarrhea and dyskinesia in jilin province in china. antigenic and genomic characterizations of the virus (isolate phev-jlsp09) were based on multiplex pcr and negative staining electron microscopy and sequence analysis of the hemagglutinin-esterase (he) gene. these piglets were diagnosed with porcine hemagglutinating encephalomyelitis virus (phev).necropsy was performed on the ... | 2011 | 21418610 |
| [research on types of salmonella in some animals in jilin province]. | some animals were examined for the presence of salmonella in jilin province. the rate of salmonella detection was 537 strains from 846 snakes samples (63.5%); 136 strains from 648 frog samples (20%); 185 strains from 369 pig and chick samples (50%). all isolated salmonella comprised of two subgenus (sii and siiib). serologically, groups 04, 07, 08, 09, 01, 03, 19, 03, 10, 016, 035, 043, 048, 050, 058 and 061 were identified and the isolates were classified into 13 groups and 24 serotypes. two of ... | 1997 | 10325636 |
| isolation and characterization of shiga toxin-producing escherichia coli o157:h7 from beef, pork and cattle fecal samples in changchun, china. | meat samples and fecal specimens from adult cattle were collected in changchun, china and were examined for presence of shiga toxin-producing escherichia coli (stec) serogroup o157. stec o157 strains were isolated from 2 (5%) of 40 beef, 1 (3.3%) of 30 pork, and 3 (1.7%) of 176 adult cattle fecal samples. the strains belonged to phage types (pt) 4, 8, or 47. two beef strains and a strain previously isolated from a patient in shandong, china, were pt-4 and showed a similar pfge pattern, suggestin ... | 2002 | 12499691 |
| isolation and identification of a bovine viral diarrhea virus from sika deer in china. | bovine viral diarrhea virus (bvdv) infections continue to cause significantly losses in the deer population. better isolation and identification of bvdv from sika deer may contribute significantly to the development of prophylactic therapeutic, and diagnostic reagents as well as help in prevention and control of bvdv. however, isolation and identification of bvdv from sika deer is seldom reported in literature. in this study, we collected some samples according to clinical sign of bvdv to isolat ... | 2011 | 21352530 |
| reassortant between human-like h3n2 and avian h5 subtype influenza a viruses in pigs: a potential public health risk. | human-like h3n2 influenza viruses have repeatedly been transmitted to domestic pigs in different regions of the world, but it is still uncertain whether any of these variants could become established in pig populations. the fact that different subtypes of influenza viruses have been detected in pigs makes them an ideal candidate for the genesis of a possible reassortant virus with both human and avian origins. however, the determination of whether pigs can act as a "mixing vessel" for a possible ... | 2010 | 20830295 |
| [gis-based analysis of the land suitability for manure application in the northeastern provinces]. | as an important industrial and grain production base of china, livestock and poultry industry have been rapidly developed in the northeastern provinces. with the rapid increasing amount of animal production, how to handle the huge amount of animal manure has become a critical issue for local government. a quantitative analysis based on geographic information system (gis) combining the biophysical, environmental, social and economic factors was applied to determine the land suitability for manure ... | 2010 | 20527197 |
| isolation and characterization of the first chinese strain of porcine teschovirus-8. | investigations were carried out to identify the causal agent of acute diarrhea, respiratory distress, and death of pigs on a swine farm in jilin province, northern china. only porcine teschovirus (ptv, designated as ptv-8 jilin/2003) was isolated from samples of organs. the presence of ptv was confirmed by the production of a specific cytopathic effect on susceptible cells and by the results of the immunoperoxidase monolayer assay (ipma), polymerase chain reaction, and electron microscopy. other ... | 2010 | 20362007 |
| toxoplasma gondii infection in farmed wild boars (sus scrofa) in three cities of northeast china. | the apicomplexan protozoan parasite toxoplasma gondii is a widely distributed etiological agent of foodborne illness. this parasite can cause production losses in livestock and serious disease in humans through consumption of contaminated meat. pig meat is the most likely source of human infection, and wild boars may play a role in the transmission of t. gondii by serving as a reservoir host. this study aimed to investigate the seroprevalence of antibodies to t. gondii among farmed wild boars in ... | 2017 | 28387529 |
| development of an immunochromatographic strip for serological diagnosis of porcine hemagglutinating encephalomyelitis virus. | an immunochromatographic strip was developed for the detection of an antibody against porcine hemagglutinating encephalomyelitis virus (phev). colloidal gold-labeled rabbit anti-pig immunoglobulin g (igg) was used as the detection reagent, and the phev recombinant antigens and goat anti-rabbit igg were coated on the prototype strip and the control lines, respectively. the immunochromatographic strip was capable of specifically detecting phev antibodies in serum with a hemagglutination inhibition ... | 2011 | 21398449 |
| li meixiang leads women to become well-off. integrated approach. | this article describes the experiences of a family planning worker in meihekou city, jilin province, in implementing the integrated approach to family planning. madam li meixiang is credited with being an outstanding example in improving program implementation. the new approach has helped people accept a smaller family size norm. madam li in 1981 married and shared in the responsibility of supporting a six-member family and raising her own daughter. their success in eliminating debt and impr ... | 1996 | 12291341 |