| bradyrhizobium iriomotense sp. nov., isolated from a tumor-like root of the legume entada koshunensis from iriomote island in japan. | a polyphasic study was performed to determine the taxonomic position of strain ek05(t) isolated from a root-outgrowth of entada koshunensis, a legume available in okinawa, japan. phylogenetic analysis of the 16s rrna gene showed that the strain belongs to the genus bradyrhizobium. subsequent multilocus sequence analysis with its, glnii, reca, gyrb, and atpd sequences revealed that the isolate represents a distinct evolutionary lineage within the genus bradyrhizobium. dna-dna hybridization indica ... | 2008 | 18540091 |
| phylogenetic diversity of indigenous cowpea bradyrhizobia from soils in japan based on sequence analysis of the 16s-23s rrna internal transcribed spacer (its) region. | cowpea [vigna unguiculata (l.) walp.] is an important legume crop and yet its rhizobia have not been well characterized in many areas. in the present study, sequence analysis of the bacterial 16s-23s rrna internal transcribed spacer (its) region was performed to characterize genetically 76 indigenous cowpea rhizobia from five different geographic regions (okinawa, miyazaki, kyoto, fukushima and hokkaido) of japan. the sequence analysis clustered all isolates in the genus bradyrhizobium. they wer ... | 2011 | 21498019 |