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relationship between clinical signs of upper respiratory tract disease and antibodies to mycoplasma agassizii in desert tortoises from nevada.plasma samples collected in 1990 from free-ranging desert tortoises (gopherus agassizii) with and without clinical signs of upper respiratory tract disease (urtd) from las vegas valley, clark county, nevada (usa), were tested by enzyme-linked immunosorbent assay (elisa) for antibodies to mycoplasma agassizii, a causative agent of urtd. the relationship between clinical signs and elisa test results was evaluated. of the 144 tortoises tested, 45 (31%) had clinical signs while 72 (50%) were seropos ...19979131556
upper respiratory tract disease and mycoplasmosis in desert tortoises from nevada.a population of desert tortoises (gopherus agassizii) at yucca mountain (nevada, usa) was monitored during four sampling periods using enzyme-linked immunosorbent assays (elisa) to determine the percentage of individuals that had been exposed to mycoplasma agassizii, a causative agent of upper respiratory tract disease. respiratory tract disease has been considered a significant factor in the decline of desert tortoise populations in the mojave desert (usa). few differences between sexes in elis ...19979391959
quantitative pcr method for detection of mycoplasma spp. dna in nasal lavage samples from the desert tortoise (gopherus agassizii).mycoplasma agassizii and m. testudineum have been associated with upper respiratory tract disease (urtd) in the threatened desert tortoise (gopherus agassizii). because microbiological culture methods have proven difficult to employ in wild desert tortoises, our goal was to develop a sensitive and specific qpcr method for detecting and quantifying mycoplasma dna in nasal lavage fluid collected in the field. primers for 16s ribosomal rna gene sequences specific for m. agassizii and m. testudineum ...201121565225
mycoplasma agassizii sp. nov., isolated from the upper respiratory tract of the desert tortoise (gopherus agassizii) and the gopher tortoise (gopherus polyphemus).biochemical, serological and molecular genetic studies were performed on seven mycoplasma isolates that were recovered from the upper respiratory tract of clinically ill desert tortoises. the isolates were serologically related to each other but serologically distinct from previously described species. unique mycoplasma species-specific 16s rrna nucleotide sequences were found in the proposed type strain. the name mycoplasma agassizii is proposed for these isolates. the type strain is ps6t (= at ...200111321087
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