Publications

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nested pcr-linked capillary electrophoresis and single-strand conformation polymorphisms for detection of macrolide-resistant mycoplasma pneumoniae in beijing, china.mycoplasma pneumoniae is usually susceptible to macrolides, but macrolide-resistant strains have been found frequently in recent years. mutations in domain v of the 23s rrna gene of m. pneumoniae interfere with the binding of macrolides to rrna and mediate macrolide resistance. in this study, we developed a rapid and inexpensive method that combines nested pcr (npcr), single-strand conformation polymorphisms (sscps), and capillary electrophoresis (ce) to detect macrolide-resistant mutants direct ...201020861333
[an outbreak of mycoplasma pneumoniae pneumonia in a kindergarten].to study the epidemiological and clinical features of the mycoplasma pneumoniae pneumonia (mpp) that occurred in a single class of a kindergarten in beijing in july 2006.200919534926
wu and ki polyomavirus present in the respiratory tract of children, but not in immunocompetent adults.recently, two new polyomaviruses (pyv), termed wupyv and kipyv, were identified in respiratory tract specimens from children with acute respiratory tract infections (artis). however, their roles in the disease have not been determined.200818790667
a multiplex pcr-based reverse line blot hybridization (mpcr/rlb) assay for detection of bacterial respiratory pathogens in children with pneumonia.to develop and evaluate a novel method for simultaneous identification of 12 potential bacterial pathogens in children with community-acquired pneumonia.200818085683
[study on the pathogens correlated to sexually transmitted diseases in 285 pre-pubertal girls with vulvovaginitis in beijing].to study the relationship between vulvovaginitis in pre-pubertal girls and pathogens as chlamydia trachomatis (ct), n. gonorrhoeae (ng), mycoplasma, ureaplasma urealyticum (uu), mycoplasma hominis (mh), m. genitalium (mg), m. fermentans (mf) and m. penetrans (mpe), as well as to find out the proportion of mycoplasma which is correlated to sexually transmitted diseases (std) and aids. methods vulvae swab specimens from 285 pre-pubertal girls with vulvovaginitis (case group) and 128 healthy girls ...200718080562
chlamydia pneumoniae and mycoplasma pneumoniae in young children from china with community-acquired pneumonia.eighty-five cases community-acquired pneumonia (cap) in children 5 years or younger, confirmed by chest x-ray, and 185 age-matched control patients with diarrhea or dermatitis from the outpatient department at beijing children's hospital were enrolled into this study. nasopharyngeal swab specimens were obtained from all subjects. real-time pcr-based fluorescence assays were performed for chlamydia pneumoniae and mycoplasma pneumoniae. a nested pcr was also run for c. pneumoniae for comparison of ...200515878436
[etiological study on an outbreak of acute respiratory infection in children in beijing].an outbreak of acute respiratory infections in children occurred in beijing from november to december, 2002. to investigate the etiological agents of affected children who were in day care centers and primary schools.200415555388
[clinical characteristics of bronchiolitis caused by human metapneumovirus in infants].the fact that the acute lower respiratory infections (alri) are associated with a newly discovered virus, human metapneumovirus (hmpv), has been shown in several studies. the authors conducted this study to understand the etiological and clinical characteristics of bronchiolitis, one of the most common alri in infants, caused by hmpv.200415189701
[prevalence of atypical pathogens in adult patients with community-acquired pneumonia in beijing].to study the pathogens in community-acquired pneumonia (cap), especially the prevalence of atypical pathogens.200414989822
cross-sectional study on their reproductive health status of 604 female floating residents in beijing.the rapid increases in the numbers of female labor migrants ("floating residents") in cities such as beijing have created challenges for china's reproductive health care system. an exploratory study conducted in beijing in 1996 of 507 floating workers engaged in household management work (average age, 27.4 years) sought to gain information to facilitate the design of reproductive health services for this group. the women had resided in beijing for an average of 3.7 years, generally with their ...199912295179
relative frequency of haemophilus influenzae type b pneumonia in chinese children as evidenced by serology.it is commonly held that haemophilus influenzae pneumonia among children in asia is mostly caused by serotypes other than b (hib). if so, hib conjugate vaccines would play little role in the prevention of pneumonia. in two prospective series of children hospitalized for pneumonia in china, the causative agents were searched for with a wide panel of microbiologic assays.200212075755
seroetiology of acute lower respiratory infections among hospitalized children in beijing.little is known of the etiology of childhood acute lower respiratory infections in china, where the use of antimicrobials is indiscriminate. trials to change such a policy require etiologic data, especially on the bacteria most relevant to these common diseases.200111176567
[study on the infections of u. urealyticum, m. hominis, and c. trachomatis in patients with venereal diseases and healthy controls in three areas of china].two hundred and thiry-nine patients with venereal diseases and 264 healthy controls, from yichang, liuzhou, and beijing, were detected for the antibodies to ureaplasma urealyticum (uu). mycoplasma hominis (mh), and chlamydia trachomatis (ct) by indirect hemagglutination (iha) in 1990. the results showed that: 1) in patients with venereal diseases, the positive rates was the highest in ct (27.62%), higher in uu (17.57%), and lower in mh (4.18%); 2) the levels of antibody to ct and uu were signifi ...19921301266
Antibiotic Sensitivity of 40 Mycoplasma pneumoniae Isolates and Molecular Analysis of Macrolide Resistant Isolates from Beijing, China.Minimum inhibitory concentrations of eight antibiotics were detected with 40 Chinese Mycoplasma pneumoniae isolates. Thirty-eight isolates (95%) were macrolide resistant. Each macrolide resistant isolate harbored an A2063G or A2064G point mutation in the 23S rRNA gene. All 40 isolates (100%) were type I strains, but they might have originated from different clones.201122106216
sequence analysis of the p1 adhesin gene of mycoplasma pneumoniae in clinical isolates collected in beijing in 2008 to 2009.the p1 genes of 60 mycoplasma pneumoniae clinical isolates were sequenced and compared to previously reported p1 gene sequences. an agt trinucleotide variable-number tandem repeat was identified that ranged in copy number from 5 to 14 among the isolates. in addition, a novel p1 gene variant named 2c was identified in 6 of the isolates.201121697320
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