| polymerase chain reaction-based differential diagnosis of ancylostoma duodenale and necator americanus infections in humans in northern ghana. | we evaluated a two-step semi-nested polymerase chain reaction (pcr)-based approach for the specific detection of ancylostoma duodenale dna in human faeces. the test was used to determine to what extent this species of hookworm is present in the regions of bolgatanga and garu of northern ghana. initially, the sensitivity and specificity of the pcr were tested using a range of well-defined control samples. subsequently, a total of 378 human faecal dna samples from bolgatanga and garu were subjecte ... | 2005 | 15941421 |