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field distribution of end phenomenon-negative bovine viral diarrhea virus.field isolates of bvdv which do not show the exaltation of newcastle disease virus (end) phenomenon (end(-)) are rarely reported. in this study, 45 bvdv field isolates from cattle in hokkaido prefecture in japan were analyzed by the reverse plaque formation method, the end method and observation of cytopathic effects. end(-) virus was detected in 34 of 45 isolates (75.6%), although 35 of 45 field isolates contained end phenomenon positive virus as the predominant virus population. we propose tha ...201425649948
a case control study of bovine viral diarrhea virus (bvdv) persistent infection (pi) in betsukai, hokkaido, japan.the betsukai town office implemented bovine viral diarrhea virus (bvdv) preventive activities (i.e., vaccination and surveillance) in 2006. using bulk tank milk screening followed by individual blood tests using a reverse transcription-polymerase chain reaction (rt-pcr) method, persistent infection (pi) cattle were detected and eliminated from the population. based on data for pi cattle detected between 2006 and 2007, we conducted a case control study to find risk factors associated with the pre ...201020057177
genetic and antigenic characterization of bovine viral diarrhea viruses isolated from cattle in hokkaido, japan.in our previous study, we genetically analyzed bovine viral diarrhea viruses (bvdvs) isolated from 2000 to 2006 in japan and reported that subgenotype 1b viruses were predominant. in the present study, 766 bvdvs isolated from 2006 to 2014 in hokkaido, japan, were genetically analyzed to understand recent epidemics. phylogenetic analysis based on nucleotide sequences of the 5'-untranslated region of viral genome revealed that 766 isolates were classified as genotype 1 (bvdv-1; 544 isolates) and g ...201626400674
relationship of bovine viral diarrhea virus persistent infection to incidence of diseases on dairy farms based on bulk tank milk test by rt-pcr.the prevalence of bovine viral diarrhea virus (bvdv) in dairy herds in hokkaido, japan, was estimated by reverse transcription polymerase chain reaction (rt-pcr) using bulk tank milk samples. sixteen out of 265 dairy herds were identified as bvdv positive, and at least one persistently infected (pi) cattle was recognized in each of the positive herds except for two herds of which, owners did not agree to examine individual cows. the proportion of positive herds with a history of bvdv pi was sign ...200515737472
epidemiological survey of border disease virus among sheep from northern districts of japan.the first epidemiological survey of border disease virus (bdv) was undertaken in small ruminants in japan. ovine sera, collected from the northern prefectures of hokkaido, aomori and iwate, were examined for the presence of antibodies against bdv using the neutralization peroxidase-linked antibody test. twenty-nine (17.6%) of one hundred and sixty-five samples were seropositive for bdv. results were specific, excluding cross-reactions with bovine viral diarrhea virus (bvdv). only one sample (0.6 ...201121778667
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