| pcr and dna hybridization indicate the absence of animal filariae from vectors of onchocerca volvulus in uganda. | in order to identify onchocerca volvulus larvae from vectors, dna of filaria larvae from dissected blackflies was isolated, and a 150-bp long tandemly repeated dna sequence (0-150), which occurs in many onchocerca species, was amplified using polymerase chain reaction (pcr). subsequently, the pcr product was blotted onto a nylon membrane and hybridized with dna probes specific for o. volvulus or onchocerca ochengi. filaria larvae from 395 infected simulium neavei were examined and 259 samples pr ... | 1997 | 9406774 |