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identification of isolate-specific sporozoite proteins of cryptosporidium parvum by two-dimensional gel electrophoresis.five isolates of cryptosporidium parvum collected from human, horse, and calf sources were compared for differences in sporozoite protein patterns by using two-dimensional gel electrophoresis. silver-stained two-dimensional gels contained over 300 protein spots from detergent-solubilized sporozoites. a distinguishing 106-kilodalton peptide that shifted in isoelectric point was detected in four of the five isolates. computerized two-dimensional gel analysis was performed to obtain objective quant ...19902365452
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