rna fingerprinting of respiratory syncytial virus using ribonuclease protection. application to molecular epidemiology. | we have used the technique of ribonuclease protection to define genomic variation among circulating isolates of subgroup a respiratory syncytial (rs) virus. rnas extracted from hep-2 cells infected with strains to be analyzed were hybridized with a 32p-labeled rna probe corresponding to the rs virus g glycoprotein (a2 strain). areas of nonhomology were detected by cleavage with ribonuclease a. using this technique, multiple distinct rna cleavage patterns could be distinguished among viral isolat ... | 1989 | 2723064 |
respiratory viral infections are a distinct risk for bronchiolitis obliterans syndrome and death. | bronchiolitis obliterans syndrome (bos) is the major obstacle to long-term survival after lung transplantation, in part because its pathogenesis is poorly understood and treatment options are limited. to identify unique risk factors for bos and death, we performed a retrospective cohort study on 259 consecutive adult lung transplant recipients over a 5-year period. the demographic and clinical characteristics of this population were analyzed for an association between bos or death and potential ... | 2004 | 15130908 |
ki polyomavirus detected in respiratory tract specimens from patients in st. louis, missouri. | studies have reported the presence of ki polyomavirus (kipyv) and wu polyomavirus (wupyv) in respiratory secretions of young patients. so far, evidence has not supported a link between infections with either virus and respiratory tract disease; however, there has not been a large comparison of kipyv-infected patients to age-matched patient groups. | 2010 | 19949359 |