prevalence and antimicrobial resistance of thermophilic campylobacter spp. from cattle farms in washington state.the prevalence of thermophilic campylobacter spp. was investigated in cattle on washington state farms. a total of 350 thermophilic campylobacter isolates were isolated from 686 cattle sampled on 15 farms (eight dairies, two calf rearer farms, two feedlots, and three beef cow-calf ranches). isolate species were identified with a combination of phenotypic tests, hipo colony blot hybridization, and multiplex lpxa pcr. breakpoint resistance to four antimicrobials (ciprofloxacin, nalidixic acid, ery ...200515640184
improved protocol for isolation of campylobacter spp. from retail broiler meat and use of pulsed field gel electrophoresis for the typing of improve the detection of campylobacter spp. in retail broiler meat, a reference method (r subsamples) based on the enrichment of 25 g of meat in bolton broth at 42°c under microaerobiosis was compared with an alternative method (a subsamples) consisting in the rinsing of meat samples for 30s in buffered peptone water with antimicrobials with incubation at 42°c under aerobiosis. one piece of meat (breasts, tenderloins and thighs) was rinse in experiment 1 (a1) and two pieces in experiment 2 (a ...201323545445
prevalence of campylobacter spp., escherichia coli, and salmonella serovars in retail chicken, turkey, pork, and beef from the greater washington, d.c., area.a total of 825 samples of retail raw meats (chicken, turkey, pork, and beef) were examined for the presence of escherichia coli and salmonella serovars, and 719 of these samples were also tested for campylobacter spp. the samples were randomly obtained from 59 stores of four supermarket chains during 107 sampling visits in the greater washington, d.c., area from june 1999 to july 2000. the majority (70.7%) of chicken samples (n = 184) were contaminated with campylobacter, and a large percentage ...200111722889
dissemination of antimicrobial resistant strains of campylobacter coli and campylobacter jejuni among cattle in washington state and california.the purpose of this study was to investigate the genetic similarity of campylobacter jejuni and campylobacter coli with similar antimicrobial resistance phenotypes, isolated from cattle on different farms and at different times, in order to evaluate the possible existence of disseminated antimicrobial resistant clones. pfge after smai and kpni restriction identified 23 and 16 distinct pfge patterns among 29 c. jejuni and 66 c. coli isolates, respectively. in c. coli, 51 (77%) of the resistant is ...200717321701
seasonal occurrence of campylobacter spp. in surface waters and their correlation with standard indicator bacteria.campylobacter jejuni, campylobacter coli, and a campylobacter-like organism were isolated from a number of natural water sources in central washington, including ponds, lakes, and small mountain streams at elevations ranging from 1,460 to 5,400 feet (ca. 445 to 1,646 m) above sea level. at the two sites where extensive sampling was done, the bacteria were recovered throughout the year. generally, the recovery rates were highest in the fall and winter months and lowest during the spring and summe ...19873579268
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