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improved protocol for isolation of campylobacter spp. from retail broiler meat and use of pulsed field gel electrophoresis for the typing of isolates.to improve the detection of campylobacter spp. in retail broiler meat, a reference method (r subsamples) based on the enrichment of 25 g of meat in bolton broth at 42°c under microaerobiosis was compared with an alternative method (a subsamples) consisting in the rinsing of meat samples for 30s in buffered peptone water with antimicrobials with incubation at 42°c under aerobiosis. one piece of meat (breasts, tenderloins and thighs) was rinse in experiment 1 (a1) and two pieces in experiment 2 (a ...201323545445
typing of campylobacter jejuni and campylobacter coli isolated from live broilers and retail broiler meat by flaa-rflp, mlst, pfge and rep-pcr.we analyzed 100 campylobacter spp. isolates (c. jejuni and c. coli) from grenada, puerto rico and alabama, which were collected from live broilers or retail broiler meat. we analyzed these isolates with four molecular typing methods: restriction fragment length polymorphism of the flaa gene (flaa-rflp), multilocus sequence typing (mlst), pulsed-field gel electrophoresis (pfge), and automated repetitive extragenic palindromic polymerase chain reaction (rep-pcr) using the diversilab system. all me ...201021130125
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