| [hepatitis e virus (hev) genotype and the prevalence of anti-hev in 8 species of animals in the suburbs of beijing.]. | to investigate the prevalence of anti-hepatitis e virus (hev) and genotypes of hepatitis e virus in 8 species of animals including swine, cattle, sheep, horse, donkey, dog, chicken and duck in the suburb of beijing. | 2010 | 20302698 |
| Insulin research in China and the U.K. | Insulin has been extensively studied since it was discovered by Banting and Best in 1921. Early in 1934, Dorothy Crowfoot and John Desmond Bernal obtained the first X-ray diffraction photograph of an enzyme protein: pepsin. In 1935, they took another photograph of a protein hormone: insulin. The chemical structure of protein was unknown until the amino acid sequence of bovine insulin was solved by Fred Sanger and colleagues in 1955. In 1958, the chemical synthesis of bovine insulin started in Ch ... | 2011 | 21936809 |
| molecular characterization of mycobacterium tuberculosis complex (mtbc) isolated from cattle in northeast and northwest china. | we studied throat swabs and corresponding serum samples collected from 1067 protein purified derivative (ppd)-tuberculin skin test (tst) positive cattle from different regions of china. the 1067 throat swabs were inoculated onto modified löwenstein-jensen medium for the isolation and culture of mycobacteria. acid-fast bacilli were identified using traditional biochemical methods, polymerase chain reaction (pcr) amplification and multiplex pcr. they were distinguished as mycobacterium tuberculosi ... | 2011 | 20797738 |
| cadmium in animal production and its potential hazard on beijing and fuxin farmlands. | a random sample of pairs of animal feeds and manures were collected from 215 animal barns in beijing and fuxin regions of china. the concentrations of cd in manures and feeds ranged from non-detectable to 129.8 mg/kg dry weight and non-detectable to 31 mg/kg dry weight, respectively. the concentrations of cd in pig, dairy cow and chicken manures were positively correlated to those in their feeds. about 30% of the manure samples contained cd concentrations higher than the upper limit for use in f ... | 2010 | 20060219 |
| potential risk of zoonotic transmission from young swine to human: seroepidemiological and genetic characterization of hepatitis e virus in human and various animals in beijing, china. | the aim of this study was to further investigate the prevalence of infection and genotype of hepatitis e virus (hev) among different species of animals, people whose works are related to pigs and the general population in the suburb of beijing, china. serum and faecal samples were collected from 10 animal species and humans. anti-hev was detected by enzyme immunoassays (eia); hev rna was amplified by reverse transcription-nested polymerase chain reaction (rt-npcr) method. pcr products were clone ... | 2011 | 21914080 |
| characterization of prototheca zopfii associated with outbreak of bovine clinical mastitis in herd of beijing, china. | prototheca zopfii (p. zopfii) has become an important cause of bovine mastitis in many countries. in the present study, to better understand the occurrence of one clinical mastitis (cm) outbreak due to p. zopfii, the molecular characterization and resistance patterns of the microalga were described. p. zopfii strains were isolated from 17 of 23 quarters, which suffered cm in the outbreak, and 7 of 46 cm recovered quarters before the outbreak, as well as 2 of 75 environmental samples in the dairy ... | 2011 | 22160589 |
| detection of neospora caninum in aborted bovine fetuses and dam blood samples by nested pcr and elisa and seroprevalence in beijing and tianjin, china. | neospora caninum infection is a significant cause of abortion in cattle. we investigated the tissue distribution of n. caninum in aborted bovine fetuses and dam blood samples by a nested pcr assay, and compared the nested pcr with elisa in the diagnosis of n. caninum infection. in total, 26 aborted fetuses and 813 blood samples were collected from 8 dairy herds in beijing (n=212) and tianjin (n=601), china. fifteen fetuses (57.7%) were tested n. caninum-positive by the nested pcr. n. caninum dna ... | 2009 | 19660160 |
| potential challenges to the stop tb plan for humans in china; cattle maintain m. bovis and m. tuberculosis. | thirty-eight cows in a herd were determined to be positive for bovine tuberculosis (tb). the bacterial isolation and characterization with multiplex pcr identified six mycobacterium tuberculosis isolates. the mycobacterium bovis and m. tuberculosis infection induced comparable pathology in cattle in both gross pathology and histopathology based on the qualitative assessment of the sampled lung tissues. the spoligotyping demonstrated that cow m. tuberculosis isolates belonged to beijing family st ... | 2009 | 19056318 |
| beijing 1987: china's coming-out party. | | 2008 | 18833263 |
| [structure of livestock and variation of fecal nitrogen pollution load in china]. | based on the livestock statistical data of 31 provinces of 1997 - 2004, this paper analyzed the structure of livestock and the variation of fecal nitrogen pollution load in china. the results showed that the relationship between structure of livestock and the economic situation of different provinces was obviously significant. the nitrogen pollution load of livestock was increasing gradually from the northwest to the southeast, and could be divided into relative higher and lower parts, in line w ... | 2007 | 17674742 |
| [study on relationships between seven microsatellite loci and somatic cell score in beijing holstein cows]. | genetic variation of seven microsatellite loci bm1818, bm1258, bm1443, bm1905, bm302, bm4505 and cyp21 which were closely linked to somatic cell score (scs) was analyzed in 240 beijing holstein cows with nondenaturing polyacrylamide gel electrophoresis. allele frequencies, heterozygosity, polymorphic information content, the effective number of alleles of seven microsatellite loci were calculated. relationships between seven microsatellite loci and somatic cell score in beijing holstein cows wer ... | 2005 | 16018256 |
| characterization of class 1 integrons-mediated antibiotic resistance among calf pathogenic escherichia coli. | escherichia coli isolates from calf diarrhea cases (n=22) in the beijing surrounding region in china were characterized for disease serotype, virulence factors, antimicrobial susceptibility pattern and class 1 integrons. 59% (n=13) of the isolates were positive for the int i1 gene. the presence and genetic content of class 1 integrons in 13 e. coli isolates were examined by pcr and sequencing. sequencing analysis revealed six gene cassettes, which encoded resistance to trimethoprim (dfra1, dfra1 ... | 2005 | 15837385 |
| effect of fluoridated milk on caries in primary teeth: 21-month results. | use of fluoridated milk has been shown to be a safe and effective method to prevent caries in permanent teeth in children. however, studies on its effectiveness on caries prevention in the primary teeth are scarce. the purpose of the present study was to evaluate the effect of milk fluoridation on caries development in young chinese children. | 2003 | 12846845 |
| [a molecular epidemiologic investigation of north asia fever in scenic spots of beijing suburb]. | pcr/rflp technique was used to detect spotted fever group rickettsiae (sfgr) in ticks and small mammals collected in eleven scenic spots of beijing suburb. we not only detected rickettsia sibirica in d. sinicus and hedgehog collected nearby the museum of aviation, but also isolated two strains of sfgr from them, named as bj-95 strain and bjh-95 strain respectively. the two strains were identified as r. sibirica by sds-page, western blot and pcr/rflp. the results demonstrated the existence of hor ... | 1997 | 9812516 |
| ten-year experience with pericardial xenograft valves. | five hundred fifty-three pericardial xenografts were inserted in 520 patients during a 9-year period at the fu wai hospital in beijing. the bovine pericardial xenograft was modeled after a similar type of prosthesis manufactured by shiley inc. (irvine, calif.). the late mortality rate in this series was only 1.8% per annum and the actuarial survival rate was 73.0% +/- 12% at 10 years. there was a very acceptable low incidence of thromboembolism of 0.41% per annum without the need for permanent a ... | 1988 | 3352290 |
| greenhouse gas emissions from dairy open lot and manure stockpile in northern china: a case study. | the open lots and manure stockpiles of dairy farm are major sources of greenhouse gas (ghg) emissions in typical dairy cow housing and manure management system in china. ghg (co(2), ch(4) and n(2)o) emissions from the ground level of brick-paved open lots and uncovered manure stockpiles were estimated according to the field measurements of a typical dairy farm in beijing by closed chambers in four consecutive seasons. location variation and manure removal strategy impacts were assessed on ghg em ... | 2016 | 26891681 |
| prevalence and molecular characterization of cryptosporidium spp. and giardia duodenalis in dairy cattle in beijing, china. | 822 fecal samples from cattle in six areas of beijing were examined with microscopy for cryptosporidium oocysts and giardia cysts. the overall infection rates for cryptosporidium spp. and giardia duodenalis were 2.55% and 1.09%, respectively. cryptosporidium was only detected in calves and heifers, whereas g. duodenalis was found in all age groups. cryptosporidium spp. were characterized with a pcr-restriction fragment length polymorphism analysis and dna sequence analysis of the small subunit ( ... | 2016 | 26921041 |
| survey of nine abortifacient infectious agents in aborted bovine fetuses from dairy farms in beijing, china, by pcr. | abortion in dairy cattle causes considerable economic losses to the dairy industry. aborted fetuses and samples from the corresponding aborting dams from 12 dairy herds in beijing were tested for 9 abortifacient infectious pathogens by pcr between 2008 and 2010. from a total of 80 abortion cases collected during this period, infectious agents were detected in 45 (56.3%) cases, 22 (48.9%) of which represented co-infections with two or three infectious agents. the detected pathogens included infec ... | 2012 | 22366134 |
| genetic characterization of bovine viral diarrhea virus strains in beijing, china and innate immune responses of peripheral blood mononuclear cells in persistently infected dairy cattle. | to acquire epidemiological data on the bovine viral diarrhea virus (bvdv) and identify cattle persistently infected (pi) with this virus, 4,327 samples from holstein dairy cows were screened over a four-year period in beijing, china. eighteen bvd viruses were isolated, 12 from pi cattle. based on genetic analysis of their 5'-untranslated region (5'-utr), the 18 isolates were assigned to subgenotype bvdv-1m, 1a, 1d, 1q, and 1b. to investigate the innate immune responses in the peripheral-blood mo ... | 2015 | 26119170 |