| [investigations on the epidemiology of brucellosis in some villages (pasturelands) of su nan county, gansu province]. | during april-may 1991, by using sat. cft. and coomb's methods epidemiological surveillance on brucellosis among human beings and animals in some villages and pasturelands of su nan county was conducted. we found out that the serum brucella antibody was positive in 61 cases (19.06% out of 320 human beings) and 49 cases were diagnosed as brucellosis. we also made an investigation on 497 cattle and 1989 sheep with sat methods. the result showed that 15 sera showing a positive reaction all came from ... | 1993 | 8156577 |
| serum biochemical values and mineral element contents of tissues in yaks. | serum biochemical values and the wet weight to dry weight ratios of tissues were determined in yaks in shandan county of gansu province. the liver, kidney, heart and muscle contents of seven elements in yaks were also analysed. most serum biochemical values were similar to those of cattle, camels and sheep, but the calcium concentration was considerably above the normal range for other ruminants. the liver contained the highest concentrations of copper, manganese and cobalt and the kidney of sel ... | 1995 | 8619285 |
| sero-epidemiological surveillance of hypodermosis in yaks and cattle in north china by elisa. | a large-scale serological surveillance was conducted for hypodermosis in the north of china. a total of 4175 sera samples of yaks and cattle were collected from five provinces in north china that included xinjiang, inner mongolia, heilongjiang, jiling and gansu provinces, between 2001 and 2002, and were examined for anti-hypoderma antibody by elisa. the results indicate that the naturally infested positive rates of hypoderma spp. in the above provinces were 51.77%, 27.02%, 13.00%, 6.03% and 44.4 ... | 2005 | 15817213 |
| molecular study of echinococcus in west-central china. | west-central china is an important endemic focus of both alveolar and cystic echinococcosis where several species of intermediate host are commonly infected with echinococcus granulosus and e. multilocularis . isolates of e. granulosus were collected from humans and other animals from different geographical areas of qinghai, ningxia, gansu and sichuan, and genotyped using the mitochondrial dna marker atp synthase subunit 6 gene (atp6). the sheep strain (g1 genotype) of e. granulosus was shown to ... | 2005 | 16174420 |
| first description of the endogenous life cycle of hypoderma sinense affecting yaks and cattle in china. | larvae belonging to five species of hypoderma spp. (diptera, oestridae) cause myiasis in wild and domestic ruminants that is characterized by migrations within deep tissues. in china hypodermosis is one of the most important arthropod diseases affecting ruminants and, moreover, represents a significant zoonosis, with numerous reports of hypoderma spp. affecting farmers. recently, a sixth species, hypoderma sinense pleske, has been rediscovered but the endogenous migration pathway within the host ... | 2006 | 17044884 |
| detecting and differentiating theileria sergenti and theileria sinensis in cattle and yaks by pcr based on major piroplasm surface protein (mpsp). | theileria sergenti and theileria sinensis are closely related members of benign theileria species found in cattle and yaks in china. they are morphologically indistinguishable. a polymerase chain reaction (pcr) targeting major piroplasm surface protein of t. sergenti and t. sinensis was developed in this study. the newly developed oligonucleotide primer set was able to specifically amplify the dna of t. sinensis and in conjunction with primers for t. sergenti and these two species could be detec ... | 2010 | 20685208 |
| trypanosoma (herpetosoma) grosi: first isolation from chinese striped field mouse (apodemus agrarius). | a "lewisi-like" trypanosoma parasite was isolated from the blood of chinese striped field mice (apodemus agrarius) trapped in the fields in the gannan tibet area, gansu province, china. the parasite was successfully cultivated in vitro in hl-1 medium supplemented 20% fetal bovine serum (fbs). full formed spheromastigote, metacyclic trypomastigote and trypomastigote structures were all visible in films made from the culture. a nucleotide fragment of 2159-bp length was amplified from genomic dna o ... | 2010 | 21059401 |
| 16s ribosomal dna analysis and characterization of lactic acid bacteria associated with traditional tibetan qula cheese made from yak milk. | twenty strains of lactic acid bacteria were isolated from six traditional tibetan qula cheese made from yak which were collected from northwest china, including tibet, qinghai and gansu province. these isolates were subjected to phenotypic and genetic analyses. all isolates were gram-positive and catalase-negative cocci that produced gas from glucose and formed d(-) isomer of lactate. most isolates were able to grow in de man, rogosa and sharpe (mrs) broth at ph values 3.0-9.0 and in 6.5% nacl ( ... | 2010 | 21108692 |
| characterization of a bovine viral diarrhea virus originated from cattle in gansu province, china. | a bovine viral diarrhea disease virus (bvdv) gs-4 was isolated in western china form dairy cattle with respiratory disease. genomic comparison analysis with the 5' half genome sequence encompassing the coding region of n(pro), capsid, and envelope glycoproteins showed that the gs-4 should be classified into bvdv-1b1, which is considered as one of the predominant subgenotypes found in china. this classification was confirmed by phylogenetic analysis based on e2 coding region. | 2013 | 23681710 |
| prevalence and molecular characterization of cryptosporidium spp. in dairy cattle, northwest china. | cryptosporidium spp. cause enteric diseases in a wide range of animals, including dairy cattle. however, limited information is available regarding prevalence and molecular characterization of cryptosporidium spp. in dairy cattle in gansu province and ningxia hui autonomous region (nxhar), northwest china. a total of 2945 dairy feces samples (1257 from gansu province and 1688 from nxhar) were collected between december 2012 and march 2014 and were tested by pcr amplification of the small subunit ... | 2015 | 26002827 |
| prevalence, risk factors and multilocus genotyping of giardia intestinalis in dairy cattle, northwest china. | giardia intestinalis is a cosmopolitan protozoan parasite that can infect a range of animals, including dairy cattle. as information regarding the prevalence and genotyping of g. intestinalis infection in dairy cattle in northwestern china is limited, 2,945 feces samples from 1,224 dairy cattle in gansu province and from 1,614 in ningxia hui autonomous region (nxhar) were examined between december 2012 and march 2014. the overall prevalence of g. intestinalis was 3.63% (107/2,945), with 2.63% an ... | 2016 | 26729604 |
| high incidence of oxacillin-susceptible meca-positive staphylococcus aureus (os-mrsa) associated with bovine mastitis in china. | staphylococcus aureus is a main cause of bovine mastitis and a major pathogen affecting human health. the emergence and spread of methicillin-resistant staphylococcus aureus (mrsa) has become a significant concern for both animal health and public health. this study investigated the incidence of mrsa in milk samples collected from dairy cows with clinical mastitis and characterized the mrsa isolates using antimicrobial susceptibility tests and genetic typing methods. in total, 103 s. aureus isol ... | 2014 | 24523877 |
| seroprevalence and correlates of toxoplasma gondii infection in dairy cattle in northwest china. | prevalence of antibodies to toxoplasma gondii and risk factors with infection were assessed in dairy cattle from gansu province and ningxia hui autonomous region (nxhar), northwest china. in total, 1657 serum samples were collected and assayed by the modified agglutination test. the overall seroprevalence was 4.83% at a 1:100 cut-off, with titers of 1:100 in 72, 1:200 in 4, 1:400 in 4. among the risk factors examined, no statistically significant difference was observed between t. gondii seropre ... | 2015 | 26408581 |