| detection of rickettsia rickettsii and rickettsia sp. in blood clots in 24 patients from different municipalities of the state of sao paulo, brazil. | the authors detected rickettsia genus organisms using shell vial and polymerase chain reaction (pcr)/sequencing analysis in blood clots in patients suspected of having brazilian spotted fever (bsf). dna was detected using pcr with three sets of primers to access the glta, ompa, and ompb genes. sequence analysis was carried out using an automatic sequencer with bioedit software. seventy-five percent of the culture samples were positive and all samples amplified rickettsial gene fragments. to date ... | 2006 | 17114719 |
| isolation of rickettsia rhipicephali and rickettsia bellii from haemaphysalis juxtakochi ticks in the state of são paulo, brazil. | in the present study, attempts to isolate rickettsia in cell culture were performed individually in seven specimens of haemaphysalis juxtakochi ticks collected in the state of são paulo (southeastern brazil). rickettsia was successfully isolated by the shell vial technique and established in vero cell culture from six ticks (six isolates). dna extracted from infected cells of these isolates was tested by pcr and dna sequencing, using genus-specific rickettsia primers targeting the genes glta, ht ... | 2007 | 17142361 |
| ticks (acari: ixodidae) infesting wild birds in an atlantic forest area in the state of são paulo, brazil, with isolation of rickettsia from the tick amblyomma longirostre. | during field work in nazaré paulista, state of são paulo, brazil, we found 13 (56.5%) of 23 birds (mostly passeriformes) to be infested by 28 larvae and 1 nymph of amblyomma spp. two larvae were reared to the adult stage, being taxonomically identified as amblyomma parkeri fonseca and aragio, whereas five larvae and one nymph were identified as amblyomma longirostre koch. all six a. longirostre specimens were shown to be infected by rickettsia, as demonstrated by polymerase chain reaction (pcr) ... | 2008 | 18714882 |