| study of perkinsosis in the carpet shell clam tapes decussatus in galicia (nw spain). i. identification of the aetiological agent and in vitro modulation of zoosporulation by temperature and salinity. | morphological characters of zoosporulation stages and dna sequence of the internal transcribed spacer (its) region and the small subunit ribosomal rna (ssu rrna) gene confirmed that the aetiological agent of perkinsosis in the clam tapes decussatus from galicia (nw spain) was perkinsus atlanticus azevedo, 1989. in vitro modulation by temperature and salinity of the zoosporulation of the parasite was studied. the optimum temperature range for zoosporulation was 19 to 28 degrees c. the temperature ... | 2002 | 12152905 |
| study of perkinsosis in the carpet shell clam tapes decussatus in galicia (nw spain). ii. temporal pattern of disease dynamics and association with clam mortality. | temporal dynamics of the infection by perkinsus olseni in a clam (tapes decussatus) bed was studied over 5 yr (march 1996 to december 2000). diagnostic techniques were compared to assess their suitability for epizootiological purposes. a technique based on incubation of 2 gill lamellae in ray's fluid thioglycollate medium (rftm) was more sensitive, quicker and cheaper than examination of histological sections. incubation of the whole-clam soft tissues in rftm allowed detection of very light infe ... | 2005 | 16119895 |
| perkinsus olseni and p. chesapeaki detected in a survey of perkinsosis of various clam species in galicia (nw spain) using pcr-dgge as a screening tool. | a survey on perkinsosis was performed involving 15 locations scattered along the galician coast (nw spain) and four clam species with high market value (ruditapes decussatus, ruditapes philippinarum, venerupis corrugata and polititapes rhomboides). the prevalence of perkinsus parasites was estimated by pcr using genus-specific primers. the highest percentage of pcr-positive cases for perkinsosis corresponded to clams r. decussatus and v. corrugata, while lower values were detected in r. philippi ... | 2016 | 26628210 |
| variable protein profiles in extracellular products of the protistan parasite perkinsus olseni among regions of the spanish coast. | the variability of the protein expression profiling in the extracellular products (ecps) of in vitro cultured perkinsus olseni deriving from 4 regions of the spanish coast was evaluated. the regions involved were the rías of arousa and pontevedra (galicia, nw spain), carreras river (andalusia, sw spain) and delta de l'ebre (catalonia, ne spain). p. olseni in vitro clonal cultures were produced from parasite isolates from four clams from each region. proteins released by the in vitro cultured par ... | 2015 | 26555510 |
| cell proteome variability of protistan mollusc parasite perkinsus olseni among regions of the spanish coast. | we evaluated the proteome variability of in vitro-cultured perkinsus olseni cells deriving from 4 regions of the spanish coast: the rías of arousa and pontevedra (galicia, nw spain), carreras river in huelva (andalusia, sw spain) and delta de l'ebre (catalonia, ne spain). p. olseni in vitro clonal cultures were produced starting from parasite isolates from 4 individual clams from each region. those clonal cultures were used to extract cell proteins, which were separated by 2-dimensional (2d) ele ... | 2015 | 25850402 |