| molecular detection and characterization of spotted fever group rickettsiae in taiwan. | rickettsioses are emerging infectious diseases caused by rickettsiae in association with arthropods. we report the detection of spotted fever group rickettsiae (sfgr) in taiwan using molecular methods. phylogenetic analyses of the 17-kd protein and citrate synthase (glta) genes showed that sfgr twkm01 detected in rhipicephalus haemaphysaloides ticks was most similar to rickettsia rhipicephali. three twkm01 isolates were obtained from three individual r. haemaphysaloides ticks. small, intracellul ... | 2007 | 17984347 |
| isolation and identification of a novel spotted fever group rickettsia, strain ig-1, from ixodes granulatus ticks collected on orchid island (lanyu), taiwan. | a novel species of spotted fever group (sfg) rickettsia, rickettsia spp. ig-1, was identified and isolated from adult ixodes granulatus ticks collected from orchid island (lanyu), an islet located in southeastern taiwan. serum samples collected from the rodent host rattus losea and sfg-positive human serum reacted with ig-1 using an immunofluorescence assay (ifa). pairwise nucleotide sequence analysis of 16s rrna (rrs), glta, ompa, ompb, and sca4 shows that ig-1 belongs to sfg rickettsiae and ha ... | 2008 | 18689633 |
| molecular analysis of ixodes granulatus, a possible vector tick for borrelia burgdorferi sensu lato in taiwan. | the genetic identity of ixodes granulatus ticks was determined for the first time in taiwan. the phylogenetic relationships were analyzed by comparing the sequences of mitochondrial 16s ribosomal dna gene obtained from 19 strains of ticks representing seven species of ixodes and two outgroup species (rhipicephalus sanguineus and haemaphysalis inermis). four major clades could be easily distinguished by neighbour-joining analysis and were congruent by maximum-parsimony method. all these i. granul ... | 2009 | 19184580 |
| first detection and molecular identification of borrelia burgdorferi-like spirochetes in ixodes granulatus ticks collected on kinmen island of taiwan. | borrelia burgdorferi-like spirochetes were detected for the first time in ixodes granulatus ticks collected in taiwan. the genetic identities of these detected spirochetes were determined by analyzing the gene sequences amplified by a genospecies-specific polymerase chain reaction assay based on the outer surface protein a (ospa) gene of b. burgdorferi sensu lato. phylogenetic analysis reveals that the sequence similarity of these spirochetes, ranging from 90.5% to 98.7% is highly homogeneous wi ... | 2009 | 19270287 |
| molecular detection of borrelia valaisiana-related spirochetes from ixodes granulatus ticks in taiwan. | borrelia valaisiana-related spirochetes were detected for the first time in ixodes granulatus ticks collected in taiwan. the genetic identities of these detected spirochetes were determined by analyzing the gene sequences amplified by a genospecies-specific polymerase chain reaction assay based on the outer surface protein a (ospa) gene of b. burgdorferi sensu lato. phylogenetic relationships were analyzed by comparing the sequences of ospa gene obtained from 35 strains of borrelia spirochetes r ... | 2010 | 20585840 |
| the presence of borrelia valaisiana-related genospecies in ticks and a rodent in taiwan. | a field survey was conducted to investigate the presence of borrelia burgdorferi sensu lato (s.l.) in six counties of taiwan. spirochetes were successfully isolated from one rodent ear sample out of 485 rodent ears and 53 live, fed tick (ixodes granulatus) samples. the spirochetes were confirmed to be b. burgdorferi s.l. by real-time pcr. in addition, 23 of 113 tick samples were tested positive for borrelia dna according to real-time pcr. the borrelia isolate from the rodent and the 23 borrelia ... | 2010 | 21221950 |
| prevalence and molecular identification of borrelia spirochetes in ixodes granulatus ticks collected from rattus losea on kinmen island of taiwan. | ixodes granulatus is widely distributed in various countries of southeast asia and taiwan. although this tick species is presumed to be the vector for the enzoonotic transmission of borrelia spirochetes in the taiwan area, the prevalence of infection and genetic diversity of borrelia spirochetes harbored by this tick species need to be further determined. | 2012 | 22882955 |
| widespread rickettsia spp. infections in ticks (acari: ixodoidea) in taiwan. | ticks are second to mosquitoes as the most important disease vectors, and recent decades have witnessed the emergence of many novel tick-borne rickettsial diseases, but systematic surveys of ticks and tick-borne rickettsioses are generally lacking in asia. we collected and identified ticks from small mammal hosts between 2006 and 2010 in different parts of taiwan. rickettsia spp. infections in ticks were identified by targeting ompb and glta genes with nested polymerase chain reaction. in total, ... | 2015 | 26336223 |
| species identification of ixodes granulatus (acari: ixodidae) based on internal transcribed spacer 2 (its2) sequences. | to investigate the genetic specificity of ixodes granulatus ticks collected from taiwan, the genetic identities and phylogenetic relationships were analyzed by comparing the sequences of the internal transcribed spacer 2 (its2) region obtained from 27 strains of ticks representing twelve species of ixodes. five major clades can be easily distinguished by neighbour-joining analysis and were congruent by maximum-parsimony method. all these i. granulatus ticks collected from taiwan and japan were g ... | 2011 | 21153754 |