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rickettsia species infecting amblyomma cooperi ticks from an area in the state of são paulo, brazil, where brazilian spotted fever is endemic.owing to the potential role of the tick amblyomma cooperi in the enzootic cycle of rickettsia rickettsii, the etiologic agent of brazilian spotted fever (bsf), this study evaluated infection by rickettsia species in a. cooperi ticks collected from an area in brazil where bsf is endemic. among a total of 40 a. cooperi adult ticks collected in an area of bsf endemicity in the state of são paulo, pcr analysis detected dna of rickettsia bellii in 16 ticks (40%), and 3 other ticks (7.5%) were positiv ...200414715737
[rickettsiae detection in amblyomma ticks (acari: ixodidae) collected in the urban area of campinas city, sp].the city of campinas is located in an endemic area for brazilian spotted fever in são paulo state, where several cases have recently occurred. capybaras have been associated with the cycle of this disease, for they present positive serology and serve as host for ticks of the genus amblyomma, the main vectors of brazilian spotted fever. ticks were collected both from capybaras and from the vegetation in the city park lago do café, located in the urban area of campinas city, sp, a site associated ...200616501770
isolation of rickettsia rickettsii and rickettsia bellii in cell culture from the tick amblyomma aureolatum in brazil.brazilian spotted fever (bsf) is a highly lethal disease caused by rickettsia rickettsii. in the present study, rickettsial infection was evaluated in 669 amblyomma aureolatum adult ticks collected from naturally infested dogs in taiaçupeba, a bsf-endemic area in the state of são paulo. ten (1.49%) ticks were infected with rickettsia bellii, and 6 (0.89%) ticks were infected with r. rickettsii. both rickettsia species were isolated and established in vero cell cultures. the rickettsia isolates w ...200617114770
isolation of rickettsia rhipicephali and rickettsia bellii from haemaphysalis juxtakochi ticks in the state of são paulo, brazil.in the present study, attempts to isolate rickettsia in cell culture were performed individually in seven specimens of haemaphysalis juxtakochi ticks collected in the state of são paulo (southeastern brazil). rickettsia was successfully isolated by the shell vial technique and established in vero cell culture from six ticks (six isolates). dna extracted from infected cells of these isolates was tested by pcr and dna sequencing, using genus-specific rickettsia primers targeting the genes glta, ht ...200717142361
rickettsia infection in five areas of the state of são paulo, brazil.this study investigated rickettsial infection in animals, humans, ticks, and fleas collected in five areas of the state of são paulo. eight flea species (adoratopsylla antiquorum antiquorum, ctenocephalides felis felis, polygenis atopus, polygenis rimatus, polygenis roberti roberti, polygenis tripus, rhopalopsyllus lugubris, and rhopalopsyllus lutzi lutzi), and five tick species (amblyomma aureolatum, amblyomma cajennense, amblyomma dubitatum, ixodes loricatus, and rhipicephalus sanguineus) were ...200718094887
rickettsial infection in capybaras (hydrochoerus hydrochaeris) from são paulo, brazil: serological evidence for infection by rickettsia bellii and rickettsia parkeri.in brazil, capybaras (hydrochoerus hydrochaeris) are important hosts for amblyomma ticks, which in turn can transmit rickettsiae to humans and animals. therefore, capybaras are potential sentinels for rickettsial infection.200718320102
[survey of rickettsia spp in the ticks amblyomma cajennense and amblyomma dubitatum in the state of são paulo].the presence of rickettsial infection was surveyed in 3,545 amblyomma cajennense ticks and 2,666 amblyomma dubitatum ticks. using the hemolymph test, polymerase chain reaction and isolation of rickettsia in cell cultures, all of the amblyomma cajennense were negative, whereas 634 (23.8%) of the amblyomma dubitatum ticks were shown to be infected with rickettsia bellii.200919684990
natural infection, transovarial transmission, and transstadial survival of rickettsia bellii in the tick ixodes loricatus (acari: ixodidae) from brazil.an ixodes loricatus engorged female, infected with rickettsia bellii, was collected from an opossum (didelphis aurita) in mogi das cruzes, são paulo state, brazil. two consecutive laboratory tick generations (f(1) and f(2)) reared from this single engorged female were evaluated for rickettsia infection by polymerase chain reaction (pcr) targeting specific rickettsia genes. immature ticks fed on naïve wistar rats (rattus norvegicus) and adult ticks fed on opossum (d. aurita), both free of ticks a ...200617114723
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