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hepatitis a virus detection in oysters (crassostrea gigas) in santa catarina state, brazil, by reverse transcription-polymerase chain reaction.shellfish are readily contaminated with viruses present in water containing sewage because of the concentration effect of filter feeding. hepatitis a virus (hav) is the main cause of acute hepatitis worldwide and may lead to severe illness or even death. it is transmitted through fecal and oral routes and causes widespread endemic and asymptomatic infections in young children. here we describe a method for the detection of hav rna in shellfish involving the extraction of total rna from oyster me ...200312636311
depuration dynamics of oysters (crassostrea gigas) artificially contaminated by salmonella enterica serovar typhimurium.the state of santa catarina produces the greatest quantity of edible mollusks in brazil. to guarantee sanitary qualify, mollusk cultures should be monitored for contamination by pathogenic microorganisms. a self-purification or "depuration" system that eliminates salmonella enterica serovar typhimurium contamination from oysters has been developed and evaluated. the depuration process occurred within a closed system, in which 1000 l of water was recirculated for 24 h. the water was sterilized wi ...200717280712
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