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restriction fragment length polymorphism analysis of 16s rdna and low molecular weight rna profiling of rhizobial isolates from shrubby legumes endemic to the canary islands.thirty-six strains of slow-growing rhizobia isolated from nodules of four woody legumes endemic to the canary islands were characterised by 16s rdna pcr-rflp analyses (ardra) and lmw rna profiling, and compared with reference strains representing bradyrhizobium japonicum, b. elkanii, b. liaoningense, and two unclassified bradyrhizobium sp. (lupinus) strains. both techniques showed similar results, indicating the existence of three genotypes among the canarian isolates. analysis of the combined r ...200011108022
bradyrhizobium canariense sp. nov., an acid-tolerant endosymbiont that nodulates endemic genistoid legumes (papilionoideae: genisteae) from the canary islands, along with bradyrhizobium japonicum bv. genistearum, bradyrhizobium genospecies alpha and bradyrhizobium genospecies beta.highly diverse bradyrhizobium strains nodulate genistoid legumes (brooms) in the canary islands, morocco, spain and the americas. phylogenetic analyses of its, atpd, glnii and reca sequences revealed that these isolates represent at least four distinct evolutionary lineages within the genus, namely bradyrhizobium japonicum and three unnamed genospecies. dna-dna hybridization experiments confirmed that one of the latter represents a new taxonomic species for which the name bradyrhizobium canarien ...200515774626
strains nodulating lupinus albus on different continents belong to several new chromosomal and symbiotic lineages within bradyrhizobium.in this work we analysed different chromosomal and symbiotic markers in rhizobial strains nodulating lupinus albus (white lupin) in several continents. collectively the analysis of their rrs and atpd genes, and 16s-23s intergenic spacers (its), showed that they belong to at least four chromosomal lineages within the genus bradyrhizobium. most isolates from the canary islands (near to the african continent) grouped with some strains isolated on mainland spain and were identified as bradyrhizobium ...201020135225
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