| rapid detection of potato y potyvirus in potato farms of kermanshah using rt-pcr amplification of the p1-protease gene and its cloning. | in this study, the rt-pcr method was used to detect the y virus in potato tubers and leaves. samples suspicious of virus infection with symptoms of virus infection were gathered from farms in kermanshah and placed in plastic bags and kept at -80 degrees c temperature in order to maintain the rna of the virus until extraction. the extraction and purification of rna were carried out using tri-reagent kit. one of the virus genes is the p1 protease gene which codes a proteinase enzyme. this enzyme p ... | 2008 | 18817251 |