identification of pvy-n on potato and tobacco in tehran and mazandaran provinces. | during two growing seasons in years of 2003 and 2004 potato and tobacco of virus infected plants were collected from fields in tehran (damavand) and mazandaran (behshahr) provinces. serological methods of tas-elisa and diba were performed by using pvy antiserum (dsmz - plant virus collection; germany) but only pvy was detected. the strain of samples was determined by using mab of potato virus y (as-0403/1; dsmz; germany). the molecular weight of the virus coat protein was approximately 34 kda in ... | 2005 | 16637208 |
purification of potato virus x and preparation of its antiserum. | potato virus x (pvx) isolated from the potato leaf and tuber samples which were collected from various fields in damavand and ardabil. the initial isolations of the virus were made from potato by mechanical inoculation on gomphrena globosa l. and chenopodium spp. that produce local lesion, and then it causes mosaic on nicotiana spp. and datura stramonium l. an isolate of the virus inoculated to nicotiana glutinosa l. and it was maintained throughout the work. sap from infected n. glutinosa was i ... | 2005 | 16637210 |
determination of the races of potato virus x and its host rang in karaj, damavand and ardabil, iran. | potato virus x (pvx) is found commonly in potato-growing areas, worldwide. it is an economically important virus which causes losses in tuber yield of approximate 5 to 15 percent. in a 2 year survey, potato leaf and tuber samples were collected from various fields in damavand and karaj. the initial isolations from potato were made by mechanical inoculation first to gomphrena globosa l. and later to dartura stramonium l. it was not transmitted by 2 species of cuscuta but transmitted mechamically. ... | 2005 | 16637211 |
potato virus yisolated from pepper fields in tehran province. | potato virus y was known as the main cause of yellowing and vein necrosis of pepper in tehran province, using double antibody sandwich elisa (das-elisa). biological properties including host range of the isolate was determined after biological purification. host range studies showed that pepper isolate of pvy caused vein clearing and mosaic symptoms on datura metel and capsicum annum, mosaic on nicotiana tabacum cv. white barley, n. tabacum cv. samsun and n. rustica but didn't show any symptoms ... | 2006 | 17390898 |