a universal pcr primer to detect members of the potyviridae and its use to examine the taxonomic status of several members of the family.a universal primer (sprimer: 5'-ggx aay aay agy ggx caz cc-3', x = a, g, c or t; y = t or c; z = a or g), designed from the consensus sequences that code for the conserved sequence gnnsgqp in the nib region of members of the family potyviridae, was used to amplify by rt-pcr the 3'-terminal genome regions from infected plant samples representing 21 different viruses in the family. sequencing of some of the fragments (c. 1.7 kb) showed that the type strain (attc pv-107) of oat necrotic mottle viru ...200111402861
characterisation of some carla- and potyviruses from bulb crops in china. brief report.conserved carla- and potyvirus primers were used in rt-pcr to amplify virus fragments from garlic and other bulb crops in china and the fragments were subsequently sequenced and compared in phylogenetic analyses. garlic plants from henan, hubei, jiangsu, shangdong and yunnan provinces all contained at least one isolate each of garlic latent virus (genus carlavirus), onion yellow dwarf virus (oydv, genus potyvirus) and leek yellow stripe virus (lysv, genus potyvirus). the complete sequence of a z ...200211890533
sequence analysis demonstrates that onion yellow dwarf virus isolates from china contain a p3 region much larger than other potyviruses.the complete sequence of an isolate of onion yellow dwarf virus (oydv) from yuhang, zhejiang province, china, was determined. it was 10538 nts in length and was predicted to encode a polyprotein 3403 amino acids (aa) long with a calculated m(r) of 385.1 kda. the predicted p3 protein (530 aa) was larger than that of any of the potyviruses sequenced to date (344-378 aa). the additional sequence occurs at the n-terminus of the protein, does not represent a duplication from elsewhere in the oydv gen ...200312756621
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