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[first detection of kashmir bee virus in hesse, germany].we gathered dead bees of 56 hessian bee colonies following a sudden collapse during winter 2002/03. viral rna was purified from ten dead bees per sample. kashmir bee virus (kbv) was detected by use of a rt-pcr protocol. 13 samples were positive for kbv. the pcr amplicon was sequenced. a blast genbank search clearly identified the hessian amplicon as a kbv fragment. similarities of more than 85% were found. phylogenetic analysis revealed a close genetic relationship of the hessian isolate to an i ...200414964117
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