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regional variations in the population structure of pseudomonas syringae pathovar phaseolicola from spain are revealed by typing with pmei pulsed-field gel electrophoresis, plasmid profiling and virulence gene complement.one hundred and twenty pathogenic isolates of pseudomonas syringae pv. phaseolicola recovered in spain were subjected to biochemical and genomic typing, and investigated for virulence gene complement. fifty-six were recovered from common beans (phaseolus vulgaris) of the type granja asturiana, grown in a northern spanish region (asturias), and 64 from other common beans cultured in the neighbouring region of castilla y león. typing by pmei digestion followed by pulsed-field gel electrophoresis r ...201020167625
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