| phylogenetic analyses of genes from south african lpai viruses isolated in 2004 from wild aquatic birds suggests introduction by eurasian migrants. | in 2004, south africa experienced its first recorded outbreak of a highly pathogenic notifiable avian influenza (hpnai) viral strain of the h5n2 subtype in ostriches in the eastern cape province. the traditional ostrich-farming areas in the western cape province report almost yearly outbreaks of low pathogenicity avian influenza (lpai) in ostriches, which is attributed to introduction by wild birds and certain climatic patterns. during the winter of 2004, lpai h3n8, h4n8, h5n2 and h5n1 avian inf ... | 2006 | 16447511 |
| a rapid and sensitive real-time reverse transcription pcr for the pathotyping of south african h5n2 avian influenza viruses. | a fluorescence resonance energy transfer (fret) real-time reverse-transcription (rrt-pcr) assay was developed that distinguishes stains of south african and european highly pathogenic (hpai) from low pathogenicity (lpai) h5 avian influenza viruses in the absence of virus isolation, irrespective of the length of insertion at the hemagglutinin cleavage site (h0). the assay was used to pathotype h5-type viruses detected by rrt-pcr in ostrich tracheal swabs collected during the 2006 hpai h5n2 outbre ... | 2008 | 19294990 |
| pathobiology of highly pathogenic avian influenza virus h5n2 infection in juvenile ostriches from south africa. | in 2011, over 35,000 ostriches were slaughtered in the oudtshoorn district of the western cape province of south africa following the diagnosis of highly pathogenic avian influenza virus h5n2. we describe the pathology and virus distribution via immunohistochemistry in juvenile birds that died rapidly in this outbreak after showing signs of depression and weakness. associated sialic acid (sa) receptor distribution in uninfected birds is also described. at necropsy, enlarged spleens, swollen live ... | 2012 | 23402120 |
| molecular analysis of the 2011 hpai h5n2 outbreak in ostriches, south africa. | the third outbreak of highly pathogenic avian influenza (hpai) h5n2 in less than seven years affected ostriches of south africa's western cape during 2011. twenty farms tested pcr positive for the presence of hpai h5n2 between march and november 2011. three hpai h5n2 (ai2114, ai2214, ai2512) and 1 h1n2 (ai2887) viruses were isolated during this period, but h6n2 and h1n2 infections of ostriches were also confirmed by pcr. hpai h5n2 isolate ai2114 produced an intravenous pathogenicity index (ivpi) ... | 2012 | 23402106 |
| risk factors for seropositivity to h5 avian influenza virus in ostrich farms in the western cape province, south africa. | in a 2005 serological survey, carried out in response to an outbreak of h5n2 avian influenza (ai) in ostriches in the eastern cape province, 16.3% of ostrich farms in the western cape province of south africa were found to be seropositive to h5 ai virus. we subsequently carried out a questionnaire-based census survey on all available registered western cape ostrich farms that still existed at the end of 2005 (367 farms, of which 82 were seropositive), in order to identify risk factors associated ... | 2008 | 18486977 |