| development of recombinant coat protein antibody based ic-rt-pcr for detection and discrimination of sugarcane streak mosaic virus isolates from southern india. | sugarcane streak mosaic virus (scsmv), causes mosaic disease of sugarcane and is thought to belong to a new undescribed genus in the family potyviridae. the coat protein (cp) gene from the andhra pradesh (ap) isolate of scsmv (scsmv-ap) was cloned and expressed in escherichia coli. the recombinant coat protein was used to raise high quality antiserum. the cp antiserum was used to develop an immunocapture reverse transcription-polymerase chain reaction (ic-rt-pcr) based assay for the detection an ... | 2003 | 12756623 |