| semi-nested pcr detection of babesia orientalis in its natural hosts rhipicephalus haemaphysaloides and buffalo. | babesiosis has recently been recognized as an emerging infectious disease of buffalo in china. in order to investigate the epidemiology and enzootic potential of this parasite in hubei province, we sought to develop a semi-nested pcr to detect babesia orientalis in buffalo and the potential tick vector-rhipicephalus haemaphysaloides by amplifying a specific 257bp fragment of b. orientalis 18s rrna gene. the practical limit of detection showed that it had high sensitivity and an approximate paras ... | 2007 | 17005323 |