| expression of the blomia tropicalis paramyosin blo t 11 and its immunodominant peptide in insect cells. | blo t 11, a dust-mite (blomia tropicalis) paramyosin, is an allergen with significant ige reactivity that has potential as a diagnostic/therapeutic reagent for house-dust-mite allergy. the present study describes the successful expression of blo t 11 and its immunodominant peptide fd in insect cells using a baculovirus expression system. the blo t 11 and fd genes were cloned into the pmelbaca vector and the resulting vectors were co-transfected into sf9 insect cells with bac-n-blue dna. plaque a ... | 2006 | 16584386 |
| sensitization to dust mites in children with allergic rhinitis in singapore: does it matter if you scratch while you sneeze? | previously published data established blomia tropicalis, as the major source of allergic sensitization in asthmatic children in tropical singapore. objective to define the prevalence, clinical characteristics and risk factors of species-specific mite sensitization in paediatric allergic rhinitis (ar) patients in this unique environment. | 2005 | 15836750 |
| sensitization profiles of malaysian and singaporean subjects to allergens from dermatophagoides pteronyssinus and blomia tropicalis. | the house dust mites dermatophagoides pteronyssinus (der p) and blomia tropicalis (blo t) are the most common house dust mite species in southeast asia. to date, there have only been a few studies on the sensitization profile of the general populations in southeast asia to house dust mites. the aim of this study was to determine the profiles of der p and blo t sensitization among singaporean and malaysian subjects. | 2003 | 14646382 |
| identification of shared and unique immunoglobulin e epitopes of the highly conserved tropomyosins in blomia tropicalis and dermatophagoides pteronyssinus. | tropomyosin belongs to a class of highly conserved proteins in invertebrates and vertebrates. the invertebrate tropomyosins are allergenic in man with high ige cross-reactivity and have been therefore referred to as pan-allergens. | 2002 | 12190660 |
| pattern of sensitization to common environmental allergens amongst atopic singapore children in the first 3 years of life. | this study was aimed to investigate the sensitization pattern to a range of common allergens in young singaporean children. a cross-sectional study involving 75 children aged below 3 years was carried out. they presented between december 1995 and april 2000 with symptoms of asthma, rhinitis, eczema, or food allergy. their levels of allergen-specific serum ige to a panel of foods (egg white, milk, soy protein, shrimp, wheat and peanut), pet dander, dust mites and cockroaches were measured with ph ... | 2001 | 12009071 |
| sensitization to blomia tropicalis and dermatophagoides pteronyssinus-a comparative study between singapore and taiwan. | blomia tropicalis (bt) and dermatophagoides pteronyssinus (dp) are the predominant domestic mites species in singapore and taiwan. this study aims to characterize and compare the mite sensitization profiles in both countries. skin prick tests were performed on 203 singaporeans with dp and bt crude extracts. in vitro ige and igg4 reactivity to extracts and specific allergens (der p 1, der p 2 der p 5 and blo t 5) were determined by immunoassays. approximately 91% of the tested singaporeans were s ... | 1999 | 10697257 |
| mite amylase from blomia tropicalis (blo t 4): differential allergenicity linked to geographical regions. | blomia tropicalis is an important domestic dust mite in the tropics and subtropics. this study describes cdna cloning of the group 4 allergen of b. tropicalis, and the evaluation of the sensitization of this allergen in atopic populations from 2 geographic regions. | 2009 | 19033729 |
| mite component-specific ige repertoire and phenotypes of allergic disease in childhood: the tropical perspective. | sensitization to perennial aeroallergens correlates with the risk of persistent asthma (as) in children. in tropical singapore, multiple codominant species of mites abound in the indoor environment, and preferential species-specific sensitization has been associated with different phenotypes of allergic disease. we investigated the pattern of mite component-specific ige (mcsige) in children with different phenotypes of clinical allergic disease in an environment with multiple mite species exposu ... | 2011 | 21332797 |
| house dust mite fauna of tropical singapore. | sensitization to house dust mites is very common in the tropics. this study evaluated the dust mite fauna in singaporean homes. | 1999 | 10051724 |
| prevalence and distribution of indoor allergens in singapore. | immediate hypersensitivity to indoor allergens is known to be associated with allergic asthma. this study evaluated the prevalence and distribution of six indoor allergens in 956 dust samples obtained from homes, childcare centres, schools, and a hospital in tropical singapore. seasonality of mite allergens was also assessed. | 1997 | 9291283 |
| saliva and serum eosinophil cationic protein in asthmatic children and adolescents with and without allergic sensitization. | saliva eosinophil cationic protein (ecp) is higher in asthmatic adults than in healthy ones. however, its relationship with asthma severity, other atopic conditions and allergic sensitization have not been reported. saliva collection is painless, readily acceptable to children and parents, and can be a potentially useful body fluid to measure asthma biomarkers. we recruited 102 physician-diagnosed young asthmatic subjects from outpatient clinics with change in fev(1) > or = 12% with inhaled salb ... | 2010 | 20100023 |