Publications

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the effects of culture-site, depth, season, and stock source on the prevalence of marteilia refringens in cultured mussels (mytilus galloprovincialis lmk.) from galicia, spain.samples of a marteiliad parasite in mytilus galloprovincialis lmk. from nw spain were identified as marteilia refringens, using light and electron microscopy. the most important lesions in the mussel tissues were found in the digestive tubules at the moment of the release of the sporangia, resulting in a breakage of the epithelial cells of the digestive tubules. the plasmodia of a marteilia-like organism were also found in the gills, accompanied by a severe host reaction in 1 of the 4,200 examin ...19957776120
detection of cryptosporidium oocysts in bivalve molluscs destined for human consumption.clams (dosinia exoleta, ruditapes philippinarum, venerupis pullastra, venerupis rhomboideus, venus verrucosa), mussels (mytilus galloprovincialis), and oysters (ostrea edulis) were tested for the presence of cryptosporidium sp. oocysts using various stain techniques and a commercially available kit containing fluorescein isothiocyanate-conjugated monoclonal antibodies. all molluscs were harvested in northwest spain (galicia) except for r. philippinarum, which was from italy, and 1 of the 6 oyste ...200010958471
temporal trends of cd, cu, hg, pb and zn in mussel (mytilus galloprovincialis) from the spanish north-atlantic coast 1991-1999.temporal trends for heavy metals (cd, cu, hg, pb and zn) in mussel (mytilus galloprovincialis) from the galician and cantabrian areas in spain, where samples were yearly collected from 1991 to 1999, are presented. this study was carried out by the centro oceanográfico de vigo of the instituto español de oceanografia (i.e.o.) as part of the spanish contribution to the joint assessment and monitoring programme (jamp) of the ospar convention. the experimental work and subsequent statistical treatme ...200211991527
assessment of coastal marine pollution in galicia (nw iberian peninsula); metal concentrations in seawater, sediments and mussels (mytilus galloprovincialis) versus embryo-larval bioassays using paracentrotus lividus and ciona intestinalis.sediments from three galician rias were tested for toxicity using sea-urchin and ascidian sediment elutriate embryo-larval bioassays. trace metal contents in seawater, sediments and mussels were also determined and subjected to multidimensional scaling methods which grouped stations according to chemical contamination. high metal contents were found in seawater, sediments and mussels from the ria of pontevedra, and moderate levels were detected in the ria of vigo and ria of arousa. the results r ...200312860437
the first identification of azaspiracids in shellfish from france and spain.incidents of human intoxications throughout europe, following the consumption of mussels have been attributed to azaspiracid poisoning (azp). although first discovered in ireland, the search for the causative toxins, named azaspiracids, in other european countries has now led to the first discovery of these toxins in shellfish from france and spain. separation of the toxins, azaspiracid (aza1) and analogues, aza2 and aza3, was achieved using isocratic reversed-phase liquid chromatography coupled ...200312893067
interspecific variation of metal concentrations in three bivalve mollusks from galicia.there has been growing concern about the inflow of metals to the coastal areas because they can be toxic to aquatic and human life. some studies have demonstrated the existence of species-specific differences in the metal concentrations of mollusks. we compared metal concentrations between mytilus galloprovincialis, used as a water quality indicator, and two other bivalve species collected for human consumption (venerupis pullastra and cerastoderma edule) in different locations on the galician c ...200415386128
proteomic approach to probe for larval proteins of the mussel mytilus galloprovincialis.a proteomic approach was used to search for larval proteins specific to the mussel mytilus galloprovincialis from galicia in northwest spain. the study included both a comparative analysis, through two-dimensional electrophoresis, of protein expression maps of the larvae of the mussel and of 5 abundant and commercially important bivalve species from the region (ostrea edulis, cerastoderma edule, pecten maximus, tapes decussatus, and venarupis pullastra) and subsequent mass spectrometric analysis ...200515976943
distribution of aliphatic compounds in bivalve mollusks from galicia after the prestige oil spill: spatial and temporal trends.the content and distribution of n-alkane (c8-c35) and isoprenoid (pristane and phytane) hydrocarbons were investigated in two species of bivalve mollusk, mussel (mytilus galloprovincialis) and cockle (cerastoderma edule), collected at different points of the galicia littoral zone during the period from december 2002 to february 2003 (after the prestige oil spill). samples were analyzed by high-resolution gas chromatography equipped with a flame ionization detector. the highest levels were found ...200616253223
cell and tissue biomarkers in mussel, and histopathology in hake and anchovy from bay of biscay after the prestige oil spill (monitoring campaign 2003).in order to assess the biological effects of the prestige oil spill (pos), mussels (mytilus galloprovincialis), european hake (merluccius merluccius) and european anchovy (engraulis encrasicolus) were sampled between april and september 2003 in various geographical areas of bay of biscay: galicia, central cantabrian and east cantabrian. in mussels, several cell and tissue biomarkers were measured: peroxisome proliferation as induction of acyl-coa oxidase (aox) activity, lysosomal responses as ch ...200616271373
cryptosporidium contamination in harvesting areas of bivalve molluscs.cryptosporidium contamination was evaluated in areas in galicia (northwestern spain) where bivalve molluscs are harvested. galicia is the main mussel-producing region in europe. data were collected on water contamination of effluents that are discharged into these areas. cryptosporidium spp. were detected by immunofluorescence microscopy and molecular methods in 71% of the river water samples (n = 7), 64% of raw sewage samples (n = 11), 50% of effluents from wastewater treatment plants (n = 16), ...200616416917
spatial and temporal trends of petroleum hydrocarbons in wild mussels from the galician coast (nw spain) affected by the prestige oil spill.polycyclic aromatic hydrocarbons (pahs) were determined in tissues of wild mussels (mytilus galloprovincialis) from the galicia coast (nw spain) in order to assess the extent of the environmental impact caused by the prestige oil spill (november 13, 2002). three sampling campaigns were carried out in february, june and november 2003 at 24 stations along the galicia coast, from la guardia (pontevedra) to ribadeo (lugo). the spatial distribution of pahs found in the first sampling period, clearly ...200616860851
free amino acid composition in juveniles of mytilus galloprovincialis: spatial variability after prestige oil spill.composition of free amino acids (faa) in juveniles of mytilus galloprovincialis was analysed along a large geographical coastline area in galicia (nw spain). individuals were sampled in february 2003, three months after the prestige oil spill. pollution values at sampling time were reported as polycyclic aromatic hydrocarbons (pahs) concentrations in soft tissues of individuals and varied between the highest amount observed in carrumeiro mussels (502 ng/g dw) and the lowest in pindo mussels (196 ...200616876449
coccomyxa sp. (chlorophyta: chlorococcales), a new pathogen in mussels (mytilus galloprovincialis) of vigo estuary (galicia, nw spain).in this work, we describe the occurrence of irregular shaped green aggregations in the mantle, gill filaments, adductor muscle, visceral mass and haemolymph of wild mussels (mytilus galloprovincialis) collected from the vigo estuary (galicia, nw spain). microscopic examination of these masses revealed that they consist of intracellular green algae which are spherical to oval in shape, 5 microm in length and 3 microm in width, without flagella and with a smooth surface. the algal cells present a ...200919686756
integrated assessment of water quality of the costa da morte (galicia, nw spain) by means of mussel chemical, biochemical and physiological parameters.the aim of this study was to assess environmental quality at some of the sites most severely affected by the prestige oil spill off 2 years after the spillage (april and november 2004). for this purpose analyses of polycyclic aromatic hydrocarbons (pah) and several biochemical (antioxidant enzymes catalase, glutathione reductase, glutathione peroxidase, superoxide dismutase and dt-diaphorase and lipid peroxidation) and physiological [scope for growth (sfg)] biomarkers were determined on wild mus ...201020033486
genotyping of hepatitis a virus detected in bivalve shellfish in galicia (nw spain).hepatitis a virus (hav) represents a significant public health problem due to its high persistence in the environment and its transmission through contaminated water and food. bivalve shellfish are filter feeders that can bioaccumulate human pathogens found in contaminated waters, their consumption being a potential cause of hepatitis a outbreaks. in this work, cultured and wild bivalve shellfish from the ría de vigo (galicia, nw spain) were analysed for the presence and genotyping of hav. a tot ...201020057087
occurrence and distribution of metals in mussels from the cantabrian coast.from february 2004 to april 2006, the concentrations of more than 10 metals in mussels (mytilus galloprovincialis) were measured in 10 stations from galicia to the basque country in 8 different times of sampling. the mean highest concentrations are found for zn [226.8 microg/g, dry weight (d.w.)] followed by mn, as, cu, and co (between 10 and 25 microg/g d.w.). lower mean concentrations are found for se, cr, pb, v, and ni (between 2 and 8 microg/g d.w.) and the lowest are for cd, sn, hg, and sb ...201020143053
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