| quantitative determination of four constituents of tinospora sps. by a reversed-phase hplc-uv-dad method. broad-based studies revealing variation in content of four secondary metabolites in the plant from different eco-geographical regions of india. | this paper describes the separation and quantitation of important markers, such as 20beta-hydroxyecdysone, tinosporaside, cordioside, and columbin, present in three species of tinospora viz, t. cordifolia, t. malabrica, and t. crispa. a reverse-phase (rp) high-performance liquid chromatography (hplc)-uv-diode array detection (dad) method employing gradient elution is thus developed. the marker compounds isolated from 70% ethanolic extract of t. cordfolia by repeated column chromatography are ide ... | 2006 | 16959127 |